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Plant Physiol, November 1999, Vol. 121, pp. 805-812
Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the
NADH-Glutamate Synthase Gene in Rice Cell Cultures1
Naoya
Hirose and
Tomoyuki
Yamaya*
Laboratory of Plant Cell Biochemistry, Graduate School of
Agricultural Science, Tohoku University, 1-1
Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
Okadaic acid (OKA), a potent and
specific inhibitor of protein serine/threonine phosphatases 1 and 2A,
induced the accumulation of NADH-glutamate synthase (GOGAT) mRNA within
4 h in rice (Oryza sativa L.) cell cultures. In
contrast to the transient accumulation of NADH-GOGAT mRNA by
NH4+, OKA caused a continuous accumulation for
at least 24 h. The induction of NADH-GOGAT mRNA by OKA was not
inhibited in the presence of methionine sulfoximine, which inhibited
the NH4+-induced accumulation of mRNA. These
results suggest that the OKA-sensitive protein phosphatase is involved
in the regulation of NADH-GOGAT gene expression and probably plays a
role in the signal transduction pathway downstream from
NH4+, although a signal transduction pathway
other than that of nitrogen sensing could be responsible. Nuclear
run-on assays demonstrated that the accumulation of NADH-GOGAT mRNA
induced by the supply of either NH4+ or
OKA was mainly regulated at the transcription level. OKA effects were synergistic to the NH4+-induced expression
of the NADH-GOGAT gene. In the presence of K-252a, a protein kinase
inhibitor, the accumulation of NADH-GOGAT mRNA induced by either
NH4+ or OKA was reduced. The possible roles of
protein phosphatases in the regulation of NADH-GOGAT gene expression
are discussed.
1
This work was supported in part by the Program
of Research for the Future from the Japan Society for the Promotion of
Science (JSPS-RFTF96L00604) and in part by a Grant-in Aid for
Scientific Research on Priority Areas from the Ministry of Education,
Science and Culture of Japan (nos. 09274101 and 09274102).
*
Corresponding author; e-mail tyamaya{at}biochem.tohoku.ac.jp; fax
81-22-717-8787.
© 1999 American Society of Plant Physiologists
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