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Plant Physiol, November 1999, Vol. 121, pp. 849-856
Modulation of Rubisco Activity during the Diurnal Phases of the
Crassulacean Acid Metabolism Plant Kalanchoë
daigremontiana1
Kate
Maxwell,*
Anne M.
Borland,
Richard P.
Haslam,
Brent R.
Helliker,2
Andrew
Roberts, and
Howard
Griffiths
Environmental and Molecular Plant Physiology Laboratory, King
George VI Building, Department of Agricultural and Environmental
Science, The University, Newcastle upon Tyne NE1 7RU, United Kingdom
The
regulation of Rubisco activity was investigated under high, constant
photosynthetic photon flux density during the diurnal phases of
Crassulacean acid metabolism in Kalanchoë
daigremontiana Hamet et Perr. During phase I, a significant
period of nocturnal, C4-mediated CO2 fixation
was observed, with the generated malic acid being decarboxylated the
following day (phase III). Two periods of daytime atmospheric
CO2 fixation occurred at the beginning (phase II,
C4-C3 carboxylation) and end (phase IV,
C3-C4 carboxylation) of the day. During the
1st h of the photoperiod, when phosphoenolpyruvate carboxylase was still active, the highest rates of atmospheric CO2 uptake were observed, coincident with the lowest rates
of electron transport and minimal Rubisco activity. Over the next 1 to
2 h of phase II, carbamylation increased rapidly during an initial
period of decarboxylation. Maximal carbamylation (70%-80%) was
reached 2 h into phase III and was maintained under conditions of
elevated CO2 resulting from malic acid decarboxylation.
Initial and total Rubisco activity increased throughout phase III, with maximal activity achieved 9 h into the photoperiod at the
beginning of phase IV, as atmospheric CO2 uptake
recommenced. We suggest that the increased enzyme activity supports
assimilation under CO2-limited conditions at the start of
phase IV. The data indicate that Rubisco activity is modulated in-line
with intracellular CO2 supply during the daytime phases of
Crassulacean acid metabolism.
1
The Natural Environment Research Council (NERC)
provided support to K.M. (small grant no. GR8/03663), R.P.H. (UK
studentship no. GT4/95/232), and A.R. (small grant no. GR9/2869). K.M.
is in receipt of a Royal Society University Research Fellowship.
2
Present address: Department of Biology,
University of Utah, Salt Lake City, UT 84112.
*
Corresponding author; e-mail kate.maxwell{at}newcastle.ac.uk; fax
44-191-222-5228.
© 1999 American Society of Plant Physiologists
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