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Plant Physiol, November 1999, Vol. 121, pp. 857-869
Molecular and Biochemical Characterization of the Involvement of
Cyclin-Dependent Kinase A during the Early Development of Tomato
Fruit1
Jérôme
Joubès,
Thi-Hai
Phan,
Daniel
Just,
Christophe
Rothan,
Catherine
Bergounioux,
Philippe
Raymond, and
Christian
Chevalier*
Unité de Physiologie Végétale, Institut de
Biologie Végétale Moléculaire, Centre de Recherche
Institut National de la Recherche Agronomique-Bordeaux, BP 81, 33883 Villenave d'Ornon cedex, France (J.J., D.J., C.R., P.R., C.C.); and
Institut de Biotechnologie des Plantes, Université Paris-sud,
Bâtiment. 630, 91405 Orsay cedex, France (T.-H.P.,
C.B.)
Following fruit set, the early
development of tomato (Lycopersicon esculentum Mill.)
fruit comprises two distinct phases: a cell division phase and a
consecutive phase of cell expansion until the onset of ripening. In
this study, we analyzed cytological and molecular changes
characterizing these early phases of tomato fruit development. First we
investigated the spatial and temporal regulation of the mitotic
activity during fruit development. The DNA content of isolated nuclei
from the different fruit tissues was determined by flow cytometry
analysis. The results confirm the data of mitotic activity measurements
and show that cell differentiation, leading to expanded cells, is
characterized by endoreduplication. Second, we isolated two cDNAs,
named Lyces;CDKA1 (accession no. Y17225) and
Lyces;CDKA2 (accession no. Y17226), encoding tomato
homologs of the cyclin-dependent kinase (CDK) p34cdc2.
Tomato CDKA gene expression was followed at both the
transcriptional and translational levels during fruit development. The
transcripts for Lyces;CDKA1 and
Lyces;CDKA2 and the corresponding CDKA proteins are
predominantly accumulated during the phase of cell division between
anthesis and 5 d post anthesis (DPA). In whole fruits, the maximum
CDK activity was obtained between 5 and 10 DPA. The determination
of the kinase activity using protein extracts from the different fruit
tissues was in agreement with mitotic activity analysis. It showed the
particular disappearance of the activity in the gel tissue as early as
15 DPA. The overall data of CDK activity measurements suggest a strong
post-translational regulation of CDK at the temporal and spatial levels
during early tomato fruit development.
1
This work was supported by the Ministère
de la Recherche et de la Technologie (France) (grant no. 95-5-23722
to J.J.).
*
Corresponding author; e-mail chevalie{at}bordeaux.inra.fr; fax
33-556-84-32-45.
© 1999 American Society of Plant Physiologists
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