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Plant Physiol, November 1999, Vol. 121, pp. 929-938
The t-SNARE AtVAM3p Resides on the Prevacuolar Compartment in
Arabidopsis Root Cells1
Anton A.
Sanderfoot,
Valentina
Kovaleva,
Haiyan
Zheng, and
Natasha
V.
Raikhel*
Michigan State University-Department of Energy Plant Research
Laboratory, East Lansing, Michigan, 48824-1312
Protein cargo is trafficked between
the organelles of the endomembrane system inside transport
vesicles, a process mediated by integral membrane proteins called
SNAREs (soluble N-ethylmaleimide sensitive factor
attachment protein receptors) that reside on the surface of the vesicle
(v-SNAREs) and target membrane (t-SNAREs). In examining transport of
cargo between the trans-Golgi network and the vacuole in Arabidopsis,
we have previously characterized AtPEP12p as a t-SNARE residing on the
prevacuolar compartment and AtVTI1a as a v-SNARE that interacts with
AtPEP12p. Recently, we have begun to characterize AtVAM3p, another
Arabidopsis t-SNARE that shows high sequence homology to AtPEP12p. We
have found that AtVTI1a also interacts with AtVAM3p, suggesting a role
for this t-SNARE in post-Golgi trafficking. AtVAM3p has been suggested to localize to the vacuolar membrane in Arabidopsis cells; however, using specific antisera and expression of epitope-tagged versions of
each t-SNARE, we have discovered that AtVAM3p is found on the same
prevacuolar structure as AtPEP12p in Arabidopsis root cells.
1
A.A.S. is a National Institutes of Health
Postdoctoral Fellow (no. GM18861). N.V.R. is supported by research
grants from the National Science Foundation (grant no. MCB-9507030)
and the Department of Energy (grant no. DE-FG02-91ER-20021).
*
Corresponding author; e-mail nraikhel{at}pilot.msu.edu; fax
517-353-9168.
© 1999 American Society of Plant Physiologists
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