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Plant Physiol, December 1999, Vol. 121, pp. 1081-1091
Characterization and Expression of Four Proline-Rich Cell Wall
Protein Genes in Arabidopsis Encoding Two Distinct Subsets of Multiple
Domain Proteins1
Thomas J.
Fowler,2
Christine
Bernhardt, and
Mary L.
Tierney*
Department of Botany and Agricultural Biochemistry, University of
Vermont, Burlington, Vermont 05405
We have characterized the molecular
organization and expression of four proline-rich protein genes from
Arabidopsis (AtPRPs). These genes predict two classes of cell wall
proteins based on DNA sequence identity, repetitive motifs, and domain
organization. AtPRP1 and AtPRP3
encode proteins containing an N-terminal PRP-like domain followed by a
C-terminal domain that is biased toward P, T, Y, and K. AtPRP2 and AtPRP4 represent a second,
novel group of PRP genes that encode two-domain proteins containing a
non-repetitive N-terminal domain followed by a PRP-like region rich in
P, V, K, and C. Northern hybridization analysis indicated that AtPRP1 and AtPRP3 are exclusively expressed in roots, while transcripts encoding AtPRP2 and AtPRP4 were most abundant in aerial organs of the
plant. Histochemical analyses of promoter/ -glucuronidase fusions
localized AtPRP3 expression to regions of the root
containing root hairs. AtPRP2 and AtPRP4
expression was detected in expanding leaves, stems, flowers, and
siliques. In addition, AtPRP4 expression was detected in
stipules and during the early stages of lateral root formation. These
studies support a model for involvement of PRPs in specifying
cell-type-specific wall structures, and provide the basis for a genetic
approach to dissect the function of PRPs during growth and development.
1
This research was supported by a supplement to
the National Science Foundation (grant no. IBN-9219712) and by the
U.S. Department of Agriculture (grant no. NRICGP-95-02982). C.B. was
supported by experiment station grant no. 0171655.
2
Present address: Microbiology and Molecular
Genetics Department, University of Vermont, Burlington, VT 05405.
*
Corresponding author; e-mail mtierney{at}zoo.uvm.edu; fax
802-656-0440.
© 1999 American Society of Plant Physiologists
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