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Plant Physiol, December 1999, Vol. 121, pp. 1117-1126
Molecular Dissection of the Role of Histidine in Nickel
Hyperaccumulation in Thlaspi goesingense
(Hálácsy)1
Michael W.
Persans,
Xiange
Yan,
Jean-Marc M.L.
Patnoe,
Ute
Krämer, and
David E.
Salt*
Northern Arizona University, P.O. Box 5698, Flagstaff, Arizona
86011 (M.W.P., J.-M.M.L.P., D.E.S.); Rutgers University, Waksman
Institute, Piscataway, New Jersey 08854 (X.Y.); and Fakultät
für Biologie, W 5, Universität Bielefeld, 33615 Bielefeld, Germany (U.K.)
To understand the role of free
histidine (His) in Ni hyperaccumulation in Thlaspi
goesingense, we investigated the regulation of His biosynthesis
at both the molecular and biochemical levels. Three T.
goesingense cDNAs encoding the following His biosynthetic enzymes, ATP phosphoribosyltransferase (THG1, GenBank
accession no. AF003347), imidazoleglycerol phosphate dehydratase
(THB1, GenBank accession no. AF023140), and histidinol
dehydrogenase (THD1, GenBank accession no. AF023141)
were isolated by functional complementation of Escherichia
coli His auxotrophs. Northern analysis of THG1,
THD1, and THB1 gene expression revealed
that each gene is expressed in both roots and shoots, but at the
concentrations and dosage times of Ni treatment used in this study,
these genes failed to show any regulation by Ni. We were also unable to
observe any increases in the concentration of free His in root, shoot, or xylem sap of T. goesingense in response to Ni
exposure. X-ray absorption spectroscopy of root and shoot tissue from
T. goesingense and the non-accumulator species
Thlaspi arvense revealed no major differences in the
coordination of Ni by His in these tissues. We therefore conclude that
the Ni hyperaccumulation phenotype in T. goesingense is
not determined by the overproduction of His in response to Ni.
1
This research was supported by grants from the
U.S. Department of Energy, Environmental Management Science program
(no. DE-FG07-98ER20295 to D.E.S.) and a North Atlantic Treaty
Organization fellowship awarded to U.K. by the German Academic Exchange
Service (DAAD).
*
Corresponding author; e-mail david.salt{at}nau.edu; fax
520-523-8111.
© 1999 American Society of Plant Physiologists
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