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Plant Physiol, December 1999, Vol. 121, pp. 1143-1152
Identification of a Promoter Sequence from the BETL1
Gene Cluster Able to Confer Transfer-Cell-Specific Expression in
Transgenic Maize1
Gregorio
Hueros,
Elisa
Gomez,
Nordine
Cheikh,
Janice
Edwards,
Marcia
Weldon,
Francesco
Salamini, and
Richard D.
Thompson*
Max-Planck-Institut fuer Zuechtungsforschung, Carl-von-Linné
Weg 10, Koeln, D-50829, Germany (F.S., R.D.T.); Department of Cell
Biology and Genetics, University of Alcala,
ES-28871, Alcala de Henares, Spain (G.H., E.G.); and
Monsanto, Chesterfield, St. Louis, Missouri (N.C., J.E., M.W.)
The maize (Zea mays
L.) betl1 locus, encoding a basal endosperm transfer
layer-specific protein, has been mapped and molecularly cloned in its
entirety. The locus is shown to consist of three gene copies in the
maize inbred line A69Y. To distinguish the three transcription units
from the locus name, we have termed them BETL1a, BETL1b,
and BETL1c. Two of the copies are expressed, whereas one is inactive and contains retrotransposon-like insertions in
both promoter and intron regions. Based on this information, and a
restriction site map covering 17 kb around the BETL1
locus, a DNA fragment putatively containing an active promoter sequence was identified. This fragment was tested for its ability to confer transfer-cell-specific expression in transient and stably transformed maize tissues. The transgenic maize plants obtained showed the predicted cell-type specificity of expression restricted to the basal
endosperm transfer cells, although there were minor deviations in
promoter strength and timing and accumulation of the transgene product
from the corresponding BETL-1 endogene expression pattern.
1
This work was supported by the Deutsche
Forschungsgemeinschaft (grant nos. SFB274 and SPP322 1005) and by
European Community contract no. BIO4 CT-972158.
*
Corresponding author; e-mail thompson{at}mpiz-koeln.mpg.de; fax
49-221-5062-413.
© 1999 American Society of Plant Physiologists
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