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Plant Physiol, December 1999, Vol. 121, pp. 1153-1162
Developmental and Wound-, Cold-, Desiccation-,
Ultraviolet-B-Stress-Induced Modulations in the Expression of the
Petunia Zinc Finger Transcription Factor Gene
ZPT2-21
Alexander R.
van der Krol,*
Remco M.P.
van
Poecke,3
Oscar F.J.
Vorst,2
Charlotte
Voogt,
Wessel
van Leeuwen,
Tanja W.M.
Borst-Vrensen,
Hiroshi
Takatsuji, and
Linus H.W.
van der
Plas
Laboratory of Plant Physiology, Wageningen University,
Arboretumlaan 4 6703 BD Wageningen, The Netherlands (A.R.v.d.K.,
R.M.P.v.P., O.F.J.V., C.V., W.v.L., T.W.M.B.-V., L.H.W.v.d.P.); and
Laboratory of Developmental Biology, National Institute of
Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan (H.T.)
The
ZPT2-2 gene belongs to the EPF gene
family in petunia (Petunia hybrida), which
encodes proteins with TFIIIA-type zinc-finger DNA-binding motifs. To
elucidate a possible function for ZPT2-2, we analyzed
its pattern of expression in relation to different developmental and
physiological stress signals. The activity of the ZPT2-2
promoter was analyzed using a firefly luciferase (LUC) reporter gene,
allowing for continuous measurements of transgene activity in planta.
We show that ZPT2-2::LUC is active in all plant tissues, but is strongly modulated in cotyledons upon
germination, in leaves in response to desiccation, cold treatment,
wounding, or ultraviolet-B light, and in petal tissue in response to
pollination of the stigma. Analysis of mRNA levels indicated that the
modulations in ZPT2-2::LUC expression reflect
modulations in endogenous ZPT2-2 gene expression. The
change in ZPT2-2::LUC activity by cold
treatment, wounding, desiccation, and ultraviolet-B light suggest that
the phytohormones ethylene and jasmonic acid are involved in regulating the expression of ZPT2-2. Although up-regulation of
expression of ZPT2-2 can be blocked by inhibitors of
ethylene perception, expression in plants is not induced by exogenously
applied ethylene. The application of jasmonic acid does result in an
up-regulation of gene activity and, thus, ZPT2-2 may
play a role in the realization of the jasmonic acid hormonal responses
in petunia.
1
This research has in part been paid for by the
Research School Experimental Plant Science.
2
Present address: CPRO-DLO, Droevendaalsesteeg 1, Postbus 16 6700 AA Wageningen, The Netherlands.
3
Present address: Laboratory of Entomology,
Wageningen University, Binnenhaven 7 6709 PD Wageningen, The Netherlands.
*
Corresponding author; e-mail Sander.vanderkrol{at}algem.pf.wau.nl;
fax 31-317-484740.
© 1999 American Society of Plant Physiologists
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