|
Plant Physiol, December 1999, Vol. 121, pp. 1179-1190
Auxin Is Required for Leaf Vein Pattern in
Arabidopsis1
Leslie E.
Sieburth*
Department of Biology, McGill University, 1205 Dr. Penfield Avenue,
Montreal, Quebec, Canada H3A 1B1 and Department of Biology University
of Utah, Salt Lake City, Utah 84112
To investigate possible roles of
polar auxin transport in vein patterning, cotyledon and leaf vein
patterns were compared for plants grown in medium containing polar
auxin transport inhibitors (N-1-naphthylphthalamic acid,
9-hydroxyfluorene-9-carboxylic acid, and 2,3,5-triiodobenzoic
acid) and in medium containing a less well-characterized inhibitor of
auxin-mediated processes,
2-(p-chlorophynoxy)-2-methylpropionic acid. Cotyledon
vein pattern was not affected by any inhibitor treatments, although
vein morphology was altered. In contrast, leaf vein pattern was
affected by inhibitor treatments. Growth in polar auxin transport
inhibitors resulted in leaves that lacked vascular continuity through
the petiole and had broad, loosely organized midveins, an increased
number of secondary veins, and a dense band of misshapen tracheary
elements adjacent to the leaf margin. Analysis of leaf vein pattern
developmental time courses suggested that the primary vein did not
develop in polar auxin transport inhibitor-grown plants, and that the
broad midvein observed in these seedlings resulted from the coalescence
of proximal regions of secondary veins. Possible models for leaf vein
patterning that could account for these observations are discussed.
1
This work was supported by the National Science
and Engineering Research Council of Canada (NSERC).
*
E-mail sieburth{at}biology.utah.edu; fax 801-581-4668.
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S. Christensen
Clearing Plant Tissue for Observation of Vascular Strands
CSH Protocols,
September 1, 2008;
2008(10):
pdb.prot4952 - pdb.prot4952.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. F. Degenhardt and P. C. Bonham-Smith
Arabidopsis Ribosomal Proteins RPL23aA and RPL23aB Are Differentially Targeted to the Nucleolus and Are Disparately Required for Normal Development
Plant Physiology,
May 1, 2008;
147(1):
128 - 142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Paponov, M. Paponov, W. Teale, M. Menges, S. Chakrabortee, J. A.H. Murray, and K. Palme
Comprehensive Transcriptome Analysis of Auxin Responses in Arabidopsis
Mol Plant,
March 1, 2008;
1(2):
321 - 337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Wenzel, Q. Hester, and J. Mattsson
Identification of Genes Expressed in Vascular Tissues Using NPA-Induced Vascular Overgrowth in Arabidopsis
Plant Cell Physiol.,
March 1, 2008;
49(3):
457 - 468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Sack, E. M. Dietrich, C. M. Streeter, D. Sanchez-Gomez, and N. M. Holbrook
Leaf palmate venation and vascular redundancy confer tolerance of hydraulic disruption
PNAS,
February 5, 2008;
105(5):
1567 - 1572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Wu and P. McSteen
The role of auxin transport during inflorescence development in maize (Zea mays, Poaceae)
Am. J. Botany,
November 1, 2007;
94(11):
1745 - 1755.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Petricka and T. M. Nelson
Arabidopsis Nucleolin Affects Plant Development and Patterning
Plant Physiology,
May 1, 2007;
144(1):
173 - 186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Alonso-Peral, H. Candela, J. C. del Pozo, A. Martinez-Laborda, M. R. Ponce, and J. L. Micol
The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Development,
October 1, 2006;
133(19):
3755 - 3766.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Jin, S. Li, and A. Villegas Jr.
Down-Regulation of the 26S Proteasome Subunit RPN9 Inhibits Viral Systemic Transport and Alters Plant Vascular Development
Plant Physiology,
October 1, 2006;
142(2):
651 - 661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Dimitrov and S. W. Zucker
A constant production hypothesis guides leaf venation patterning
PNAS,
June 13, 2006;
103(24):
9363 - 9368.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Sieburth, G. K. Muday, E. J. King, G. Benton, S. Kim, K. E. Metcalf, L. Meyers, E. Seamen, and J. M. Van Norman
SCARFACE Encodes an ARF-GAP That Is Required for Normal Auxin Efflux and Vein Patterning in Arabidopsis
PLANT CELL,
June 1, 2006;
18(6):
1396 - 1411.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Scheres and J. Xu
Polar auxin transport and patterning: grow with the flow
Genes & Dev.,
April 15, 2006;
20(8):
922 - 926.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Yoshida, H. Kuriyama, and H. Fukuda
Inhibition of Transdifferentiation into Tracheary Elements by Polar Auxin Transport Inhibitors Through Intracellular Auxin Depletion
Plant Cell Physiol.,
December 1, 2005;
46(12):
2019 - 2028.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-H. Lin, Y. Wang, B. Mueller-Roeber, C. A. Brearley, Z.-H. Xu, and H.-W. Xue
At5PTase13 Modulates Cotyledon Vein Development through Regulating Auxin Homeostasis
Plant Physiology,
December 1, 2005;
139(4):
1677 - 1691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Poupart, A. M. Rashotte, G. K. Muday, and C. S. Waddell
The rib1 Mutant of Arabidopsis Has Alterations in Indole-3-Butyric Acid Transport, Hypocotyl Elongation, and Root Architecture
Plant Physiology,
November 1, 2005;
139(3):
1460 - 1471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. K. Clay and T. Nelson
Arabidopsis thickvein Mutation Affects Vein Thickness and Organ Vascularization, and Resides in a Provascular Cell-Specific Spermine Synthase Involved in Vein Definition and in Polar Auxin Transport
Plant Physiology,
June 1, 2005;
138(2):
767 - 777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sawa, K. Koizumi, S. Naramoto, T. Demura, T. Ueda, A. Nakano, and H. Fukuda
DRP1A Is Responsible for Vascular Continuity Synergistically Working with VAN3 in Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
819 - 826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Koizumi, S. Naramoto, S. Sawa, N. Yahara, T. Ueda, A. Nakano, M. Sugiyama, and H. Fukuda
VAN3 ARF-GAP-mediated vesicle transport is involved in leaf vascular network formation
Development,
April 1, 2005;
132(7):
1699 - 1711.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Zgurski, R. Sharma, D. A. Bolokoski, and E. A. Schultz
Asymmetric Auxin Response Precedes Asymmetric Growth and Differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis Leaves
PLANT CELL,
January 1, 2005;
17(1):
77 - 91.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Pyo, T. Demura, and H. Fukuda
Spatial and Temporal Tracing of Vessel Differentiation in Young Arabidopsis Seedlings by the Expression of an Immature Tracheary Element-specific Promoter
Plant Cell Physiol.,
October 15, 2004;
45(10):
1529 - 1536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Navarro, N. Efremova, J. F. Golz, R. Rubiera, M. Kuckenberg, R. Castillo, O. Tietz, H. Saedler, and Z. Schwarz-Sommer
Molecular and genetic interactions between STYLOSA and GRAMINIFOLIA in the control of Antirrhinum vegetative and reproductive development
Development,
August 1, 2004;
131(15):
3649 - 3659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Scarpella, P. Francis, and T. Berleth
Stage-specific markers define early steps of procambium development in Arabidopsis leaves and correlate termination of vein formation with mesophyll differentiation
Development,
July 15, 2004;
131(14):
3445 - 3455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Carland and T. Nelson
COTYLEDON VASCULAR PATTERN2-Mediated Inositol (1,4,5) Triphosphate Signal Transduction Is Essential for Closed Venation Patterns of Arabidopsis Foliar Organs
PLANT CELL,
May 1, 2004;
16(5):
1263 - 1275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. P. d. Santos, E. Purgatto, H. Mercier, and M. S. Buckeridge
The Control of Storage Xyloglucan Mobilization in Cotyledons of Hymenaea courbaril
Plant Physiology,
May 1, 2004;
135(1):
287 - 299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zhong and Z.-H. Ye
amphivasal vascular bundle 1, a Gain-of-Function Mutation of the IFL1/REV Gene, Is Associated with Alterations in the Polarity of Leaves, Stems and Carpels
Plant Cell Physiol.,
April 15, 2004;
45(4):
369 - 385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Keller, R. Stahlberg, L. S. Barkawi, and J. D. Cohen
Long-Term Inhibition by Auxin of Leaf Blade Expansion in Bean and Arabidopsis
Plant Physiology,
March 1, 2004;
134(3):
1217 - 1226.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Perez-Perez, M. R. Ponce, and J. L. Micol
The ULTRACURVATA2 Gene of Arabidopsis Encodes an FK506-Binding Protein Involved in Auxin and Brassinosteroid Signaling
Plant Physiology,
January 1, 2004;
134(1):
101 - 117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Deyholos, G. F. Cavaness, B. Hall, E. King, J. Punwani, J. Van Norman, and L. E. Sieburth
VARICOSE, a WD-domain protein, is required for leaf blade development
Development,
December 29, 2003;
130(26):
6577 - 6588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Q. J. Steynen and E. A. Schultz
The FORKED genes are essential for distal vein meeting in Arabidopsis
Development,
October 1, 2003;
130(19):
4695 - 4708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Parker, R. Schofield, B. Sundberg, and S. Turner
Isolation of COV1, a gene involved in the regulation of vascular patterning in the stem of Arabidopsis
Development,
May 15, 2003;
130(10):
2139 - 2148.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Willemsen, J. Friml, M. Grebe, A. van den Toorn, K. Palme, and B. Scheres
Cell Polarity and PIN Protein Positioning in Arabidopsis Require STEROL METHYLTRANSFERASE1 Function
PLANT CELL,
March 1, 2003;
15(3):
612 - 625.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mattsson, W. Ckurshumova, and T. Berleth
Auxin Signaling in Arabidopsis Leaf Vascular Development
Plant Physiology,
March 1, 2003;
131(3):
1327 - 1339.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Scarpella, S. Rueb, and A. H. Meijer
The RADICLELESS1 gene is required for vascular pattern formation in rice
Development,
February 15, 2003;
130(4):
645 - 658.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Ha, G.-T. Kim, B. C. Kim, J. H. Jun, M. S. Soh, Y. Ueno, Y. Machida, H. Tsukaya, and H. G. Nam
The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Development,
January 1, 2003;
130(1):
161 - 172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Scarpella, K. J.M. Boot, S. Rueb, and A. H. Meijer
The Procambium Specification Gene Oshox1 Promotes Polar Auxin Transport Capacity and Reduces Its Sensitivity toward Inhibition
Plant Physiology,
November 1, 2002;
130(3):
1349 - 1360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Avsian-Kretchmer, J.-C. Cheng, L. Chen, E. Moctezuma, and Z. R. Sung
Indole Acetic Acid Distribution Coincides with Vascular Differentiation Pattern during Arabidopsis Leaf Ontogeny
Plant Physiology,
September 1, 2002;
130(1):
199 - 209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tobena-Santamaria, M. Bliek, K. Ljung, G. Sandberg, J. N.M. Mol, E. Souer, and R. Koes
FLOOZY of petunia is a flavin mono-oxygenase-like protein required for the specification of leaf and flower architecture
Genes & Dev.,
March 15, 2002;
16(6):
753 - 763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Evolution of developmental potential and the multiple independent origins of leaves in Paleozoic vascular plants
Paleobiology,
March 1, 2002;
28(1):
70 - 100.
|
|
|
|
|
|
|
J. H. Jun, C. M. Ha, and H. G. Nam
Involvement of the VEP1 Gene in Vascular Strand Development in Arabidopsis thaliana
Plant Cell Physiol.,
March 1, 2002;
43(3):
323 - 330.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Grozinger, E. D. Chao, H. E. Blackwell, D. Moazed, and S. L. Schreiber
Identification of a Class of Small Molecule Inhibitors of the Sirtuin Family of NAD-dependent Deacetylases by Phenotypic Screening
J. Biol. Chem.,
October 12, 2001;
276(42):
38837 - 38843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Deyholos, G Cordner, D Beebe, and L. Sieburth
The SCARFACE gene is required for cotyledon and leaf vein patterning
Development,
January 8, 2000;
127(15):
3205 - 3213.
[Abstract]
[PDF]
|
|
|
|
|
