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Plant Physiol, December 1999, Vol. 121, pp. 1207-1215
Both phyA and phyB Mediate Light-Imposed Repression of
PHYA Gene Expression in Arabidopsis1
Francisco R.
Cantón and
Peter H.
Quail*
Department of Plant and Microbial Biology, University of
California, Berkeley, California 94720 (F.R.C., P.H.Q.); and Plant Gene
Expression Center/United States Department of Agriculture-Agricultural
Research Service, 800 Buchanan Street, Albany, California 94710 (F.R.C., P.H.Q.)
The negatively photoregulated
PHYA gene has a complex promoter structure in
Arabidopsis, with three active transcription start sites. To identify
the photoreceptors responsible for regulation of this gene, and to
assess the relative roles of the three transcription start sites, we
analyzed the changes in PHYA transcript levels in
wild-type and photoreceptor mutant seedlings under various irradiation
conditions. Continuous far-red or red light exposures each induced a
significant decline in transcript levels in wild-type etiolated
seedlings. Analysis of mutants specifically lacking either phyA or phyB
protein demonstrated that these phytochromes are required for the
negative regulation induced by far-red and red light, respectively.
Ribonuclease protection experiments showed further that this negative
regulation is confined almost exclusively to the shortest, most
abundant PHYA transcript, and occurs predominantly in
shoots. By contrast, both of the other minor transcripts in shoots, and
all three transcripts in roots, exhibit near constitutive expression.
This complex expression pattern indicates that the PHYA
gene is subject to regulation by multiple signals, including environmental, developmental, and organ-specific signals.
1
This work was supported by grants from the
National Science Foundation (no. MCB 9513590); the California
Agricultural Experiment Station Project (no. CA-B*-PLB-5703 H); U.S.
Department of Agriculture, Current Research Information Service (no.
5335-21000-0010-00D); and the Spanish Ministry of Science and
Education Fellowship to F.R.C.
*
Corresponding author; e-mail quail{at}nature.berkeley.edu; fax
510-559-5678.
© 1999 American Society of Plant Physiologists
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