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Plant Physiol, December 1999, Vol. 121, pp. 1247-1255
Evidence for an Inorganic Carbon-Concentrating Mechanism in the
Symbiotic Dinoflagellate Symbiodinium sp.1
William
Leggat,
Murray R.
Badger, and
David
Yellowlees*
Biochemistry and Molecular Biology, James Cook University,
Townsville, Queensland 4811, Australia (W.L., D.Y.); and Molecular
Plant Physiology Group, Research School of Biological Sciences,
Australian National University P.O. Box 475, Canberra, Australian
Capital Territory 0200, Australia (M.R.B.)
The presence of a
carbon-concentrating mechanism in the symbiotic dinoflagellate
Symbiodinium sp. was investigated. Its existence was
postulated to explain how these algae fix inorganic carbon (Ci) efficiently despite the presence of a form II Rubisco.
When the dinoflagellates were isolated from their host, the giant clam (Tridacna gigas), CO2 uptake was found to
support the majority of net photosynthesis (45%-80%) at pH 8.0;
however, 2 d after isolation this decreased to 5% to 65%, with
HCO3 uptake supporting 35% to 95% of net
photosynthesis. Measurements of intracellular Ci
concentrations showed that levels inside the cell were between two and
seven times what would be expected from passive diffusion of
Ci into the cell. Symbiodinium also exhibits a distinct light-activated intracellular carbonic anhydrase activity. This, coupled with elevated intracellular Ci and the
ability to utilize both CO2 and
HCO3 from the medium, suggests that
Symbiodinium sp. does possess a carbon-concentrating
mechanism. However, intracellular Ci levels are not as
large as might be expected of an alga utilizing a form II Rubisco with
a poor affinity for CO2.
1
This work was supported by an Australian
Research Council grant (D.Y.) including a Ph.D. scholarship (W.L.).
*
Corresponding author; e-mail David.Yellowlees{at}jcu.edu.au; fax
61-7-47251394.
© 1999 American Society of Plant Physiologists
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