|
Plant Physiol, December 1999, Vol. 121, pp. 1273-1279
An Expansin Gene Expressed in Ripening Strawberry
Fruit1
Pedro Marcos
Civello,2
Ann L.T.
Powell,
Adnan
Sabehat,3 and
Alan B.
Bennett*
Mann Laboratory, Department of Vegetable Crops, University of
California, Davis, California 95616
Tissue
softening accompanies the ripening of many fruit and initiates the
processes of irreversible deterioration. Expansins are plant cell wall
proteins proposed to disrupt hydrogen bonds within the cell wall
polymer matrix. Expression of specific expansin genes has been observed
in tomato (Lycopersicon esculentum) meristems, expanding
tissues, and ripening fruit. It has been proposed that a tomato
ripening-regulated expansin might contribute to cell wall polymer
disassembly and fruit softening by increasing the accessibility of
specific cell wall polymers to hydrolase action. To assess whether
ripening-regulated expansins are present in all ripening fruit, we
examined expansin gene expression in strawberry (Fragaria × ananassa Duch.).
Strawberry differs significantly from tomato in that the fruit is
derived from receptacle rather than ovary tissue and strawberry is
non-climacteric. A full-length cDNA encoding a ripening-regulated
expansin, FaExp2, was isolated from strawberry fruit.
The deduced amino acid sequence of FaExp2 is most closely related to an
expansin expressed in early tomato development and to expansins
expressed in apricot fruit rather than the previously identified tomato
ripening-regulated expansin, LeExp1. Nearly all previously identified
ripening-regulated genes in strawberry are negatively regulated by
auxin. Surprisingly, FaExp2 expression was largely
unaffected by auxin. Overall, our results suggest that expansins are a
common component of ripening and that non-climacteric signals other
than auxin may coordinate the onset of ripening in strawberry.
1
This research was supported by U.S. Department
of Agriculture National Research Initiative Competitive Grants Program
(grant no. 97-35304-4627). P.M.C. was supported by a fellowship from Consejo Nacional de Investigaciones Científicas y
Técnicas, Argentina.
2
Present address: Instituto Tecnológico de
Chascomús, Camino Circunvalación Laguna Km 6, Casilla de
Correo 164, (7130) Chascomús, Argentina.
3
Present address: StePac L.A., Tefen Industrial
Park, P.O. Box 73, Tefen 24959, Israel.
*
Corresponding author; e-mail abbennett{at}ucdavis.edu; fax
530-752-4554.
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
R. E. Carey and D. J. Cosgrove
Portrait of the Expansin Superfamily in Physcomitrella patens: Comparisons with Angiosperm Expansins
Ann. Bot.,
June 1, 2007;
99(6):
1131 - 1141.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Raab, J. A. Lopez-Raez, D. Klein, J. L. Caballero, E. Moyano, W. Schwab, and J. Munoz-Blanco
FaQR, Required for the Biosynthesis of the Strawberry Flavor Compound 4-Hydroxy-2,5-Dimethyl-3(2H)-Furanone, Encodes an Enone Oxidoreductase
PLANT CELL,
April 1, 2006;
18(4):
1023 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and D. Zanin
PpEG4 is a peach endo-{beta}-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern
J. Exp. Bot.,
February 1, 2006;
57(3):
589 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and G. Casadoro
Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruits?
J. Exp. Bot.,
August 1, 2005;
56(418):
2037 - 2046.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Belfield, B. Ruperti, J. A. Roberts, and S. McQueen-Mason
Changes in expansin activity and gene expression during ethylene-promoted leaflet abscission in Sambucus nigra
J. Exp. Bot.,
March 1, 2005;
56(413):
817 - 823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Castillejo, J. I. de la Fuente, P. Iannetta, M. A. Botella, and V. Valpuesta
Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform
J. Exp. Bot.,
April 1, 2004;
55(398):
909 - 918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Zenoni, L. Reale, G. B. Tornielli, L. Lanfaloni, A. Porceddu, A. Ferrarini, C. Moretti, A. Zamboni, A. Speghini, F. Ferranti, et al.
Downregulation of the Petunia hybrida {alpha}-Expansin Gene PhEXP1 Reduces the Amount of Crystalline Cellulose in Cell Walls and Leads to Phenotypic Changes in Petal Limbs
PLANT CELL,
February 1, 2004;
16(2):
295 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-K. Lee, J. H. Ahn, S.-K. Song, Y. D. Choi, and J. S. Lee
Expression of an Expansin Gene Is Correlated with Root Elongation in Soybean
Plant Physiology,
March 1, 2003;
131(3):
985 - 997.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aharoni and A. P. O'Connell
Gene expression analysis of strawberry achene and receptacle maturation using DNA microarrays
J. Exp. Bot.,
October 1, 2002;
53(377):
2073 - 2087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Aharoni, L. C.P. Keizer, H. C. Van Den Broeck, R. Blanco-Portales, J. Munoz-Blanco, G. Bois, P. Smit, R. C.H. De Vos, and A. P. O'Connell
Novel Insight into Vascular, Stress, and Auxin-Dependent and -Independent Gene Expression Programs in Strawberry, a Non-Climacteric Fruit
Plant Physiology,
July 1, 2002;
129(3):
1019 - 1031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Jimenez-Bermudez, J. Redondo-Nevado, J. Munoz-Blanco, J. L. Caballero, J. M. Lopez-Aranda, V. Valpuesta, F. Pliego-Alfaro, M. A. Quesada, and J. A. Mercado
Manipulation of Strawberry Fruit Softening by Antisense Expression of a Pectate Lyase Gene
Plant Physiology,
February 1, 2002;
128(2):
751 - 759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Chen, P. Dahal, and K. J. Bradford
Two Tomato Expansin Genes Show Divergent Expression and Localization in Embryos during Seed Development and Germination
Plant Physiology,
November 1, 2001;
127(3):
928 - 936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, R. Spinello, A. Piovan, S. Spolaore, and G. Casadoro
{beta}-Galactosidases with a lectin-like domain are expressed in strawberry
J. Exp. Bot.,
August 1, 2001;
52(361):
1635 - 1645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. P. Harrison, S. J. McQueen-Mason, and K. Manning
Expression of six expansin genes in relation to extension activity in developing strawberry fruit
J. Exp. Bot.,
July 1, 2001;
52(360):
1437 - 1446.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Wu, R. B. Meeley, and D. J. Cosgrove
Analysis and Expression of the {alpha}-Expansin and {beta}-Expansin Gene Families in Maize
Plant Physiology,
May 1, 2001;
126(1):
222 - 232.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Li, C. P. Darley, V. Ongaro, A. Fleming, O. Schipper, S. L. Baldauf, and S. J. McQueen-Mason
Plant Expansins Are a Complex Multigene Family with an Ancient Evolutionary Origin
Plant Physiology,
March 1, 2002;
128(3):
854 - 864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
