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Plant Physiol, December 1999, Vol. 121, pp. 1309-1320
Increased Respiratory Restriction during Phosphate-Limited Growth
in Transgenic Tobacco Cells Lacking Alternative
Oxidase1
Hannah L.
Parsons,
Justine Y.H.
Yip, and
Greg C.
Vanlerberghe*
Division of Life Science and Department of Botany,
University of Toronto at Scarborough, 1265 Military Trail, Scarborough,
Ontario, Canada M1C 1A4
We found that mitochondrial
alternative oxidase (AOX) protein and the capacity for CN-resistant
respiration are dramatically increased in wild-type tobacco
(Nicotiana tabacum) suspension-cultured cells in
response to growth under P limitation, and antisense (AS8) tobacco
cells unable to induce AOX under these conditions have altered growth
and metabolism. Specifically, we found that the respiration of AS8
cells was restricted during P-limited growth, when the potential for
severe adenylate control of respiration (at the level of C supply to
the mitochondrion and/or at the level of oxidative phosphorylation) is
high due to the low cellular levels of ADP and/or inorganic P. As a
result of this respiratory restriction, AS8 cells had altered growth,
morphology, cellular composition, and patterns of respiratory C flow to
amino acid synthesis compared with wild-type cells with abundant AOX
protein. Also, AS8 cells under P limitation displayed high in vivo
rates of generation of active oxygen species compared with wild-type cells. This difference could be abolished by an uncoupler of
mitochondrial oxidative phosphorylation. Our results suggest that
induction of non-phosphorylating AOX respiration (like induction of
adenylate and inorganic P-independent pathways in glycolysis) is an
important plant metabolic adaptation to P limitation. By preventing
severe respiratory restriction, AOX acts to prevent both redirections in C metabolism and the excessive generation of harmful active oxygen
species in the mitochondrion.
1
This work was funded by a research grant from
the Natural Sciences and Engineering Research Council of Canada to
G.C.V.
*
Corresponding author; e-mail gregv{at}scar.utoronto.ca; fax
416-287-7642.
© 1999 American Society of Plant Physiologists
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