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Plant Physiol, December 1999, Vol. 121, pp. 1339-1347
Vacuolar H+-ATPase Is Expressed in Response to
Gibberellin during Tomato Seed Germination1
Michael B.
Cooley,2
Hong
Yang,
Peetambar
Dahal,
R. Alejandra
Mella,3
A. Bruce
Downie,4
Anthony M.
Haigh,5 and
Kent
J.
Bradford*
Department of Vegetable Crops, One Shields Avenue, University of
California, Davis, California 95616-8631
Completion
of germination (radicle emergence) by gibberellin (GA)-deficient
(gib-1) mutant tomato (Lycopersicon
esculentum Mill.) seeds is dependent upon exogenous GA, because
weakening of the endosperm tissue enclosing the radicle tip requires
GA. To investigate genes that may be involved in endosperm weakening or
embryo growth, differential cDNA display was used to identify mRNAs
differentially expressed in gib-1 seeds imbibed in the
presence or absence of GA4+7. Among these was a
GA-responsive mRNA encoding the 16-kD hydrophobic subunit c of the
V0 membrane sector of vacuolar H+-translocating
ATPases (V-ATPase), which we termed LVA-P1. LVA-P1 mRNA
expression in gib-1 seeds was dependent on GA and was
particularly abundant in the micropylar region prior to radicle
emergence. Both GA dependence and tissue localization of
LVA-P1 mRNA expression were confirmed directly in
individual gib-1 seeds using tissue printing.
LVA-P1 mRNA was also expressed in wild-type seeds during development and germination, independent of exogenous GA. Specific antisera detected protein subunits A and B of the cytoplasmic V1 sector of the V-ATPase holoenzyme complex in
gib-1 seeds only in the presence of GA, and expression
was localized to the micropylar region. The results suggest that
V-ATPase plays a role in GA-regulated germination of tomato seeds.
1
This research was supported by the National
Science Foundation (grant no. IBN-9407264 to K.J.B).
2
Present address: U.S. Department of
Agriculture Agricultural Research ServiceWestern Regional Center,
800 Buchanan Street, Albany, CA 94710.
3
Present address: Catedra de Fisiologia Vegetal,
Facultad de Agronomia, Universidad de Buenos Aires, Avenida San
Martin 4453, 1417 Buenos Aires, Argentina.
4
Present address: Department of Horticulture and
Landscape Architecture, N324 Agricultural Science Center-North,
University of Kentucky, Lexington, KY 40546-0091.
5
Present address: Centre for Horticulture and
Plant Sciences, Faculty of Science and Technology, University of
Western Sydney, Hawkesbury, Locked Bag 1, PO Richmond, NSW 2753, Australia.
*
Corresponding author; e-mail kjbradford{at}ucdavis.edu; fax
530-752-4554.
© 1999 American Society of Plant Physiologists
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