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Plant Physiol, December 1999, Vol. 121, pp. 1367-1374
The Redox State Regulates RNA Degradation in the Chloroplast of
Chlamydomonas reinhardtii1
Maria L.
Salvador and
Uwe
Klein*
Department of Biochemistry and Molecular Biology, University of
Valencia, Doctor Moliner 50, 46100 Valencia, Spain (M.L.S.);
and Department of Biology, University of Oslo, P.O. Box 1045, Blindern, 0316 Oslo, Norway (U.K.)
A Chlamydomonas
reinhardtii chloroplast transformant, designated MU7, carrying
a chimeric (rbcL promoter:  -glucuronidase [GUS]:
psaB 3' end) gene whose transcripts have been found
previously to be unstable in light (half-life of 20 min in light as
opposed to a half-life of 5 h in the dark), was used to study the
role of electron transport and of the redox state in the degradation of
chloroplast transcripts in the light. Blocking photosynthetic electron
transport with 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) prevented
the light-dependent breakdown of the pool of GUS transcripts in MU7
cells. Diamide, an oxidizing agent, caused a measurable delay in the
degradation of GUS transcripts in the light. The addition of
dithiothreitol (DTT), a dithiol reductant, to MU7 cells in which GUS
transcript levels were stabilized by DCMU induced degradation of GUS
transcripts. Similarly, DTT induced a decrease in the levels of GUS
transcripts when added to MU7 cells in the dark period of the
light/dark cycle, a period in which GUS transcript levels normally
increase. The levels of transcripts of endogenous chloroplast genes
were affected by DCMU and DTT in the same direction as levels of GUS
transcripts. The results suggest a regulatory role of the redox state
in the degradation of chloroplast transcripts in C.
reinhardtii.
1
The work was supported by grants from the
Dirección General de Investigacion Cientifica y Technica (grant
no. PB 95-1075 to M.L.S.) and the Norwegian Research Council (grant
no. 100946/410 to U.K.).
*
Corresponding author; e-mail uwe.klein{at}bio.uio.no; fax
47-22-85-46-64.
© 1999 American Society of Plant Physiologists
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