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Plant Physiol, January 2000, Vol. 122, pp. 189-198
A Small Heat Shock Protein Cooperates with Heat Shock Protein 70 Systems to Reactivate a Heat-Denatured Protein1
Garrett J.
Lee2 and
Elizabeth
Vierling*
Department of Biochemistry (G.J.L., E.V.) and Department of
Molecular and Cellular Biology (E.V.), The University of Arizona,
1007 E. Lowell Street, Tucson, Arizona 85721-0106.
Small heat shock proteins (sHsps) are
a diverse group of heat-induced proteins that are conserved in
prokaryotes and eukaryotes and are especially abundant in plants.
Recent in vitro data indicate that sHsps act as molecular chaperones to
prevent thermal aggregation of proteins by binding non-native
intermediates, which can then be refolded in an ATP-dependent fashion
by other chaperones. We used heat-denatured firefly luciferase (Luc)
bound to pea (Pisum sativum) Hsp18.1 as a model to
define the minimum chaperone system required for refolding of a
sHsp-bound substrate. Heat-denatured Luc bound to Hsp18.1 was
effectively refolded either with Hsc/Hsp70 from diverse eukaryotes plus
the DnaJ homologs Hdj1 and Ydj1 (maximum = 97% Luc reactivation
with kob = 1.0 × 10 2/min), or with prokaryotic Escherichia
coli DnaK plus DnaJ and GrpE (100% Luc reactivation,
kob = 11.3 × 102/min). Furthermore, we show that Hsp18.1 is more
effective in preventing Luc thermal aggregation than the Hsc70 or DnaK
systems, and that Hsp18.1 enhances the yields of refolded Luc even when other chaperones are present during heat inactivation. These findings integrate the aggregation-preventive activity of sHsps with the protein-folding activity of the Hsp70 system and define an in vitro
system for further investigation of the mechanism of sHsp action.
1
This work was supported by the National
Institutes of Health (grant no. RO1 GM42762 to E.V. and postdoctoral
fellowship no. 5F32-GM16748 to G.J.L.) and by the American Cancer
Society Faculty Research Award (no. FRA-420 to E.V.).
2
Present address: Monsanto Company, 800 N. Lindbergh Boulevard, St. Louis, MO 63167.
*
Corresponding author; e-mail vierling{at}u.arizona.edu; fax
520-621-3709.
© 2000 American Society of Plant Physiologists
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