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Plant Physiol, January 2000, Vol. 122, pp. 255-264
Wheat Granule-Bound Starch Synthase I and II Are Encoded by
Separate Genes That Are Expressed in Different Tissues1
Patricia L.
Vrinten and
Toshiki
Nakamura*
Tohoku National Agricultural Experiment Station, Akahira 4, Morioka
020-0198, Japan.
Studies of waxy mutations in
wheat and other cereals have shown that null mutations in genes
encoding granule-bound starch synthase I (GBSSI) result in amylose-free
starch in endosperm and pollen grains, whereas starch in other tissues
may contain amylose. We have isolated a cDNA from waxy wheat that
encodes GBSSII, which is thought to be responsible for the elongation of amylose chains in non-storage tissues. The deduced amino acid sequences of wheat GBSSI and GBSSII were
almost 66% identical, while those of wheat GBSSII and
potato GBSSI were 72% identical. GBSSII
was expressed in leaf, culm, and pericarp tissue, but transcripts were
not detected in endosperm tissue. In contrast, GBSSI
expression was high in endosperm tissue. The expression of
GBSSII mRNA in pericarp tissue was similar at the
midpoints of the day and night periods. The GBSSII genes
were mapped to chromosomes 2AL, 2B, and 2D, whereas
GBSSI genes are located on group 7 chromosomes. Gel-blot
analysis indicated that genes related to GBSSII also occur in barley, rice, and maize. The possible role of
GBSSII in starch synthesis is discussed.
1
This research was supported by the Science and
Technology Agency of Japan and by the Ministry of Agriculture,
Fisheries and Forestry of Japan.
*
Corresponding author; e-mail tnaka{at}tnaes.affrc.go.jp; fax
81-19-643-3514.
© 2000 American Society of Plant Physiologists
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