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Plant Physiol, January 2000, Vol. 122, pp. 57-66
Multiple Mode Regulation of a Cysteine Proteinase Gene Expression
in Rice1
Shin-Lon
Ho,2
Wu-Fu
Tong, and
Su-May
Yu2*
Department of Biology, National Taiwan Normal University, Taipei
11650, Taiwan, Republic of China (S.-L.H., W.-F.T.); and Institute of
Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, Republic
of China (S.-L.H., S.-M.Y.).
In many plants, cysteine proteinases
play essential roles in a variety of developmental and physiological
processes. In rice (Oryza sativa), REP-1 is a primary
cysteine proteinase responsible for the digestion of seed storage
proteins to provide nutrients to support the growth of young seedlings.
In the present study, the gene encoding REP-1 was isolated,
characterized, and designated as OsEP3A. An
OsEP3A-specific DNA probe was used to study the effect
of various factors on the expression of OsEP3A in
germinating seeds and vegetative tissues of rice. The expression of
OsEP3A is hormonally regulated in germinating seeds,
spatially and temporally regulated in vegetative tissues, and
nitrogen-regulated in suspension-cultured cells. The
OsEP3A promoter was linked to the coding sequence of the
reporter gene, gusA, which encodes  -glucuronidase
(GUS), and the chimeric gene was introduced into the rice genome. The OsEP3A promoter is sufficient to confer nitrogen
regulation of GUS expression in suspension-cultured cells.
Histochemical studies also indicate that the OsEP3A
promoter is sufficient to confer the hormonal regulation of GUS
expression in germinating seeds. These studies demonstrate that in rice
the REP-1 protease encoded by OsEP3A may play a role in
various physiological responses and processes, and that multiple
mechanisms regulate the expression of OsEP3A.
1
This work was supported by a grant from the
National Science Council of the Republic of China (no.
NSC81-0211-B-003-504 to W.-F.T.) and a grant from Academia Sinica
of the Republic of China (to S.-M.Y.).
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail sumay{at}ccvax.sinica.edu.tw; fax
886-2-2788-2695 or 886-2-2782-6085.
© 2000 American Society of Plant Physiologists
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