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Plant Physiol, January 2000, Vol. 122, pp. 99-106
The Ultraviolet Action Spectrum for Stomatal Opening in Broad
Bean1
William
Eisinger,
Trevor E.
Swartz,
Roberto A.
Bogomolni, and
Lincoln
Taiz*
Biology Department, Santa Clara University, Santa Clara, California
95053 (W.E.); and Chemistry Department (T.E.S., R.A.B.) and
Biology Department (L.T.), University of California, Santa
Cruz, California 95064.
The ultraviolet action spectrum for
stomatal opening was measured using epidermal peels from leaves of
broad bean (Vicia faba). The spectrum was calculated
from hyperbolic fluence response curves using 11 wavelengths ranging
from 275 to 459 nm. The action spectrum exhibits a major peak at
approximately 280 nm and a minor peak at approximately 360 nm. The
response at 280 nm is about three times greater than the response at
459 nm. Under the conditions utilized (i.e. the absence of saturating
red light), stomatal opening saturated at extremely low fluence rates:
<0.2 µmol m 2 s1 at 280 nm, and
approximately 1.0 µmol m2 s1 at 459 nm.
The threshold for blue-light-induced stomatal opening was approximately
0.02 µmol m2 s1. In light-mixing
experiments, the addition of 280 nm light to saturating 650 nm
(red) light caused additional stomatal opening, which is indicative of
separate photoreceptors. In contrast, adding 280 nm of light to
saturating 459 nm (blue) light did not increase stomatal opening,
suggesting that they both excite the same receptor. The results with
white light were similar to those with blue light. We infer that
ultraviolet light acts via the blue light photoreceptor rather than
through photosynthesis. The additional absorbance peak at 360 nm
suggests that the chromophore is either a flavin or a cis-carotenoid,
both of which exhibit peaks in this region. It is proposed that the
chromophore can be excited either directly by blue light or by
energy transferred from the protein portion of the protein-pigment
complex after it absorbs 280 nm light.
1
This research was supported by the U.S.
Department of Agriculture (grant no. 94-37100-0755 to L.T.).
*
Corresponding author; e-mail taiz{at}biology.ucsc.edu; fax
831-459-3139.
© 2000 American Society of Plant Physiologists
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