Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (25)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Hilhorst, H. W.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Hilhorst, H. W.M.
Agricola
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Hilhorst, H. W.M.

Plant Physiol, February 2000, Vol. 122, pp. 327-336

Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not1

Renato D. de Castro,2 André A.M. van Lammeren, Steven P.C. Groot, Raoul J. Bino, and Henk W.M. Hilhorst*

Laboratory of Experimental Plant Morphology and Cell Biology (R.D.d.C., A.A.M.v.L.) and Laboratory of Plant Physiology (R.D.d.C., H.W.M.H.), Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands; and Department of Reproduction Technology, Centre for Plant Breeding and Reproduction Research, CPRO, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.D.d.C., S.P.C.G., R.J.B.).

We studied cell cycle events in embryos of tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds during imbibition in water and during osmoconditioning ("priming") using both quantitative and cytological analysis of DNA synthesis and beta -tubulin accumulation. Most embryonic nuclei of dry, untreated control seeds were arrested in the G1 phase of the cell cycle. This indicated the absence of DNA synthesis (the S-phase), as confirmed by the absence of bromodeoxyuridine incorporation. In addition, beta -tubulin was not detected on western blots and microtubules were not present. During imbibition in water, DNA synthesis was activated in the radicle tip and then spread toward the cotyledons, resulting in an increase in the number of nuclei in G2. Concomitantly, beta -tubulin accumulated and was assembled into microtubular cytoskeleton networks. Both of these cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The activation of DNA synthesis and the formation of microtubular cytoskeleton networks were also observed throughout the embryo when seeds were osmoconditioned. However, this pre-activation of the cell cycle appeared to become arrested in the G2 phase since no mitosis was observed. The pre-activation of cell cycle events in osmoconditioned seeds appeared to be correlated with enhanced germination performance during re-imbibition in water.

1 This project was supported by a doctoral fellowship from Companha de Aperfeiçoamento de Pessoal de Nível Superior to R.D.d.C. (process no. 11241/92-4), Ministry of Education, Brazil.

2 Present address: Laboratório de Sementes, Departamento de Agricultura, Universidade Federal de Lavras, Cx. Postal 37, Lavras, Minas Gerais, CEP 37200-000, Brazil.

* Corresponding author; e-mail henk.hilhorst{at}algem.pf.wau.nl; fax 31-317-484740.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 ANN BOT (LOND)Home page
E. A. A. Da Silva, P. E. Toorop, A. A. M. Van Lammeren, and H. W. M. Hilhorst
ABA Inhibits Embryo Cell Expansion and Early Cell Division Events During Coffee (Coffea arabica 'Rubi') Seed Germination
Ann. Bot., September 1, 2008; 102(3): 425 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Catusse, J.-M. Strub, C. Job, A. Van Dorsselaer, and D. Job
Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression
PNAS, July 22, 2008; 105(29): 10262 - 10267.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Gendreau, S. Romaniello, S. Barad, J. Leymarie, R. Benech-Arnold, and F. Corbineau
Regulation of cell cycle activity in the embryo of barley seeds during germination as related to grain hydration
J. Exp. Bot., February 10, 2008; (2008) erm296v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Chibani, S. Ali-Rachedi, C. Job, D. Job, M. Jullien, and P. Grappin
Proteomic Analysis of Seed Dormancy in Arabidopsis
Plant Physiology, December 1, 2006; 142(4): 1493 - 1510.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. H. Masubelele, W. Dewitte, M. Menges, S. Maughan, C. Collins, R. Huntley, J. Nieuwland, S. Scofield, and J. A. H. Murray
D-type cyclins activate division in the root apex to promote seed germination in Arabidopsis
PNAS, October 25, 2005; 102(43): 15694 - 15699.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Doncheva, M. Amenos, C. Poschenrieder, and J. Barcelo
Root cell patterning: a primary target for aluminium toxicity in maize
J. Exp. Bot., April 1, 2005; 56(414): 1213 - 1220.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. d. l. P. Sanchez, S. H. Gurusinghe, K. J. Bradford, and J. M. Vazquez-Ramos
Differential response of PCNA and Cdk-A proteins and associated kinase activities to benzyladenine and abscisic acid during maize seed germination
J. Exp. Bot., February 1, 2005; 56(412): 515 - 523.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. M. Barroco, K. Van Poucke, J. H.W. Bergervoet, L. De Veylder, S. P.C. Groot, D. Inze, and G. Engler
The Role of the Cell Cycle Machinery in Resumption of Postembryonic Development
Plant Physiology, January 1, 2005; 137(1): 127 - 140.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Soeda, M. C.J.M. Konings, O. Vorst, A. M.M.L. van Houwelingen, G. M. Stoopen, C. A. Maliepaard, J. Kodde, R. J. Bino, S. P.C. Groot, and A. H.M. van der Geest
Gene Expression Programs during Brassica oleracea Seed Maturation, Osmopriming, and Germination Are Indicators of Progression of the Germination Process and the Stress Tolerance Level
Plant Physiology, January 1, 2005; 137(1): 354 - 368.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Gallardo, C. Le Signor, J. Vandekerckhove, R. D. Thompson, and J. Burstin
Proteomics of Medicago truncatula Seed Development Establishes the Time Frame of Diverse Metabolic Processes Related to Reserve Accumulation
Plant Physiology, October 1, 2003; 133(2): 664 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Jabrin, S. Ravanel, B. Gambonnet, R. Douce, and F. Rebeille
One-Carbon Metabolism in Plants. Regulation of Tetrahydrofolate Synthesis during Germination and Seedling Development
Plant Physiology, March 1, 2003; 131(3): 1431 - 1439.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Gallardo, C. Job, S. P.C. Groot, M. Puype, H. Demol, J. Vandekerckhove, and D. Job
Proteomics of Arabidopsis Seed Germination. A Comparative Study of Wild-Type and Gibberellin-Deficient Seeds
Plant Physiology, June 1, 2002; 129(2): 823 - 837.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Gallardo, C. Job, S. P.C. Groot, M. Puype, H. Demol, J. Vandekerckhove, and D. Job
Proteomic Analysis of Arabidopsis Seed Germination and Priming
Plant Physiology, June 1, 2001; 126(2): 835 - 848.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists