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Plant Physiol, February 2000, Vol. 122, pp. 447-452
Nucleoside Diphosphate Kinase Required for Coleoptile Elongation
in Rice1
Ling
Pan,
Maki
Kawai,
Akira
Yano, and
Hirofumi
Uchimiya*
Institute of Molecular and Cellular Biosciences, University of
Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan (L.P., M.K.,
A.Y., H.U.); Advanced Science Research Center, Japan Atomic Energy
Research Institute, Takasaki 370-1292, Japan (M.K., H.U.); and
National Institute of Infectious Diseases, Shinjuku, Tokyo
162-8640, Japan (A.Y.).
Although several nucleoside
diphosphate (NDP) kinase genes have been cloned in plants, little is
known about the functional significance of this enzyme during plant
growth and development. We introduced a chimeric gene encoding an
antisense RNA of NDP kinase under the control of the Arabidopsis heat
shock protein HSP81-1 promoter into rice (Oryza sativa
L.) plants using the Agrobacterium tumefaciens
transformation system. The expression of antisense RNA down-regulated
the accumulation of mRNA, resulting in reduced enzyme activity even
under the standard growth temperature (25°C) in transgenic plants.
Following heat shock treatment (37°C), NDP kinase activities in some
transgenic rice plants were more reduced than those grown under 25°C.
The comparison of the coleoptile growth under submersion showed that
cell elongation process was inhibited in antisense NDP kinase
transgenic plants, suggesting that an altered guanine nucleotide level
may be responsible for the processes.
1
This research was supported by Grants-in-Aid for
Scientific Research from the Ministry of Education, Culture and
Science, Japan, by a grant from the Rockefeller Foundation, and by
Research for the Future from the Japan Society for the Promotion of
Science (grant no. JSPS-RFTF96L00604).
*
Corresponding author; e-mail uchimiya{at}imcbns.iam.u-tokyo.ac.jp;
fax 81-3-5841-8466.
© 2000 American Society of Plant Physiologists
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