|
Plant Physiol, February 2000, Vol. 122, pp. 597-608
Metabolic Dysfunction and Unabated Respiration Precede the Loss
of Membrane Integrity during Dehydration of Germinating
Radicles1
Olivier
Leprince,*
Frans J.M.
Harren,
Julia
Buitink,
Mark
Alberda, and
Folkert A.
Hoekstra
Department of Molecular and Laser Physics, University of Nijmegen,
Toernooiveld, 6525 ED Nijmegen, The Netherlands (O.L., F.J.M.H.);
and Laboratory of Plant Physiology, Wageningen University,
Arboretumlaan 4, 6703 BD Wageningen, The Netherlands (O.L., J.B., M.A.,
F.A.H.).
This study shows that dehydration
induces imbalanced metabolism before loss of membrane integrity in
desiccation-sensitive germinated radicles. Using a photoacoustic
detection system, responses of CO2 emission and
fermentation to drying were analyzed non-invasively in
desiccation-tolerant and -intolerant radicles of cucumber
(Cucumis sativa) and pea (Pisum
sativum). Survival after drying and a membrane integrity assay
showed that desiccation tolerance was present during early imbibition
and lost in germinated radicles. However, tolerance could be re-induced
in germinated cucumber radicles by incubation in polyethylene glycol
before drying. Tolerant and polyethylene glycol (PEG)-induced tolerant
radicles exhibited a much-reduced CO2 production before
dehydration compared with desiccation-sensitive radicles. This
difference was maintained during dehydration. In desiccation-sensitive
tissues, dehydration induced an increase in the emission of
acetaldehyde and ethanol that peaked well before the loss of membrane
integrity. Acetaldehyde emission from sensitive radicles was
significantly reduced when dehydration occurred in 50% O2
instead of air. Acetaldehyde/ethanol were not detected in dehydrating
tolerant radicles of either species or in polyethylene glycol-induced
tolerant cucumber radicles. Thus, a balance between down-regulation of
metabolism during drying and O2 availability appears to be
associated with desiccation tolerance. Using Fourier transform infrared
spectroscopy, acetaldehyde was found to disturb the phase behavior of
phospholipid vesicles, suggesting that the products resulting from
imbalanced metabolism in seeds may aggravate membrane damage induced by dehydration.
1
This work was supported by the Netherlands
Organization for Scientific Research and the Technological Foundation
for Scientific Research.
*
Corresponding author; e-mail
olivier.leprince{at}guest.pf.wau.nl; fax 31-317-484740.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
J. Boudet, J. Buitink, F. A. Hoekstra, H. Rogniaux, C. Larre, P. Satour, and O. Leprince
Comparative Analysis of the Heat Stable Proteome of Radicles of Medicago truncatula Seeds during Germination Identifies Late Embryogenesis Abundant Proteins Associated with Desiccation Tolerance
Plant Physiology,
April 1, 2006;
140(4):
1418 - 1436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. A. Hoekstra
Differential Longevities in Desiccated Anhydrobiotic Plant Systems
Integr. Comp. Biol.,
November 1, 2005;
45(5):
725 - 733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. R. Faria, J. Buitink, A. A. M. van Lammeren, and H. W. M. Hilhorst
Changes in DNA and microtubules during loss and re-establishment of desiccation tolerance in germinating Medicago truncatula seeds
J. Exp. Bot.,
August 1, 2005;
56(418):
2119 - 2130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A.J. Mur, I. E. Santosa, L. J.J. Laarhoven, N. J. Holton, F. J.M. Harren, and A. R. Smith
Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars
Plant Physiology,
July 1, 2005;
138(3):
1247 - 1258.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Koster, N. Reisdorph, and J. L. Ramsay
Changing desiccation tolerance of pea embryo protoplasts during germination
J. Exp. Bot.,
June 1, 2003;
54(387):
1607 - 1614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. I. BOAMFA, P. C. RAM, M. B. JACKSON, J. REUSS, and F. J. M. HARREN
Dynamic Aspects of Alcoholic Fermentation of Rice Seedlings in Response to Anaerobiosis and to Complete Submergence: Relationship to Submergence Tolerance
Ann. Bot.,
January 2, 2003;
91(2):
279 - 290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Montero, S.M. Cristescu, J.B. Jimenez, J.M. Orea, S. te Lintel Hekkert, F.J.M. Harren, and A. Gonzalez Urena
trans-Resveratrol and Grape Disease Resistance. A Dynamical Study by High-Resolution Laser-Based Techniques
Plant Physiology,
January 1, 2003;
131(1):
129 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Quartacci, O. Glisic, B. Stevanovic, and F. Navari-Izzo
Plasma membrane lipids in the resurrection plant Ramonda serbica following dehydration and rehydration
J. Exp. Bot.,
November 1, 2002;
53(378):
2159 - 2166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Xiao and K. L. Koster
Desiccation tolerance of protoplasts isolated from pea embryos
J. Exp. Bot.,
November 1, 2001;
52(364):
2105 - 2114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Buitink, O. Leprince, and F. A. Hoekstra
Dehydration-Induced Redistribution of Amphiphilic Molecules between Cytoplasm and Lipids Is Associated with Desiccation Tolerance in Seeds
Plant Physiology,
November 1, 2000;
124(3):
1413 - 1426.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Navari-Izzo, M. F. Quartacci, C. Pinzino, N. Rascio, C. Vazzana, and C. L.M. Sgherri
Protein Dynamics in Thylakoids of the Desiccation-Tolerant Plant Boea hygroscopica during Dehydration and Rehydration
Plant Physiology,
November 1, 2000;
124(3):
1427 - 1436.
[Abstract]
[Full Text]
|
|
|
|
|
