|
Plant Physiol, March 2000, Vol. 122, pp. 635-644
Purification of a Jojoba Embryo Fatty Acyl-Coenzyme A Reductase
and Expression of Its cDNA in High Erucic Acid Rapeseed
James G.
Metz,*
Michael R.
Pollard,1
Lana
Anderson,
Thomas R.
Hayes, and
Michael W.
Lassner2
Calgene Campus, Monsanto, 1920 Fifth Street, Davis,
California 95616
The
jojoba (Simmondsia chinensis) plant produces esters of
long-chain alcohols and fatty acids (waxes) as a seed lipid energy reserve. This is in contrast to the triglycerides found in seeds of
other plants. We purified an alcohol-forming fatty acyl-coenzyme A
reductase (FAR) from developing embryos and cloned the cDNA encoding
the enzyme. Expression of a cDNA in Escherichia coli confers FAR activity upon those cells and results in the accumulation of fatty alcohols. The FAR sequence shows significant homology to an
Arabidopsis protein of unknown function that is essential for pollen
development. When the jojoba FAR cDNA is expressed in embryos of
Brassica napus, long-chain alcohols can be detected in
transmethylated seed oils. Resynthesis of the gene to reduce its A plus
T content resulted in increased levels of alcohol production. In
addition to free alcohols, novel wax esters were detected in the
transgenic seed oils. In vitro assays revealed that B.
napus embryos have an endogenous fatty acyl-coenzyme A: fatty
alcohol acyl-transferase activity that could account for this wax
synthesis. Thus, introduction of a single cDNA into B.
napus results in a redirection of a portion of seed oil
synthesis from triglycerides to waxes.
1
Present address: Department of Botany and Plant
Pathology, Michigan State University, East Lansing, MI 48824-1312.
2
Present address: Maxygen Inc., 515 Galveston
Drive, Redwood City, CA 94063.
*
Corresponding author; e-mail jim.metz{at}monsanto.com; fax
530-792-2453.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
O. Rowland, H. Zheng, S. R. Hepworth, P. Lam, R. Jetter, and L. Kunst
CER4 Encodes an Alcohol-Forming Fatty Acyl-Coenzyme A Reductase Involved in Cuticular Wax Production in Arabidopsis
Plant Physiology,
November 1, 2006;
142(3):
866 - 877.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kalscheuer, T. Stoveken, H. Luftmann, U. Malkus, R. Reichelt, and A. Steinbuchel
Neutral Lipid Biosynthesis in Engineered Escherichia coli: Jojoba Oil-Like Wax Esters and Fatty Acid Butyl Esters
Appl. Envir. Microbiol.,
February 1, 2006;
72(2):
1373 - 1379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Cheng and D. W. Russell
Mammalian Wax Biosynthesis: I. IDENTIFICATION OF TWO FATTY ACYL-COENZYME A REDUCTASES WITH DIFFERENT SUBSTRATE SPECIFICITIES AND TISSUE DISTRIBUTIONS
J. Biol. Chem.,
September 3, 2004;
279(36):
37789 - 37797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. N. Arbeitman, A. A. Fleming, M. L. Siegal, B. H. Null, and B. S. Baker
A genomic analysis of Drosophila somatic sexual differentiation and its regulation
Development,
May 1, 2004;
131(9):
2007 - 2021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.'i. Moto, T. Yoshiga, M. Yamamoto, S. Takahashi, K. Okano, T. Ando, T. Nakata, and S. Matsumoto
Pheromone gland-specific fatty-acyl reductase of the silkmoth, Bombyx mori
PNAS,
August 5, 2003;
100(16):
9156 - 9161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker, and D. J. Hawkins
DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family. PURIFICATION, CLONING, AND EXPRESSION IN INSECT CELLS OF TWO POLYPEPTIDES FROM MORTIERELLA RAMANNIANA WITH DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY
J. Biol. Chem.,
October 12, 2001;
276(42):
38862 - 38869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Lardizabal, J. G. Metz, T. Sakamoto, W. C. Hutton, M. R. Pollard, and M. W. Lassner
Purification of a Jojoba Embryo Wax Synthase, Cloning of its cDNA, and Production of High Levels of Wax in Seeds of Transgenic Arabidopsis
Plant Physiology,
March 1, 2000;
122(3):
645 - 656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
