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Plant Physiol, March 2000, Vol. 122, pp. 657-666

Expression of Aluminum-Induced Genes in Transgenic Arabidopsis Plants Can Ameliorate Aluminum Stress and/or Oxidative Stress1

Bunichi Ezaki,* Richard C. Gardner, Yuka Ezaki, and Hideaki Matsumoto

Research Institute For Bioresources, Okayama University, 2-20-1 Chuou, Kurashiki, Okayama 710-0046, Japan (B.E., H.M.); and School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand (R.C.G., Y.E.)

To examine the biological role of Al-stress-induced genes, nine genes derived from Arabidopsis, tobacco (Nicotiana tabacum L.), wheat (Triticum aestivum L.), and yeast (Saccharomyces cerevisiae) were expressed in Arabidopsis ecotype Landsberg. Lines containing eight of these genes were phenotypically normal and were tested in root elongation assays for their sensitivity to Al, Cd, Cu, Na, Zn, and to oxidative stresses. An Arabidopsis blue-copper-binding protein gene (AtBCB), a tobacco glutathione S-transferase gene (parB), a tobacco peroxidase gene (NtPox), and a tobacco GDP-dissociation inhibitor gene (NtGDI1) conferred a degree of resistance to Al. Two of these genes, AtBCB and parB, and a peroxidase gene from Arabidopsis (AtPox) also showed increased resistance to oxidative stress induced by diamide, while parB conferred resistance to Cu and Na. Al content of Al-treated root tips was reduced in the four Al-resistant plant lines compared with wild-type Ler-0, as judged by morin staining. All four Al-resistant lines also showed reduced staining of roots with 2',7'-dichloro fluorescein diacetate (H2DCFDA), an indicator of oxidative stress. We conclude that Al-induced genes can serve to protect against Al toxicity, and also provide genetic evidence for a link between Al stress and oxidative stress in plants.

1 This work was supported by the Program for Promotion of Basic Research Activities for Innovative Biosciences, the New Zealand Foundation for Research Science and Technology (no. 96-AGR-03-5253), by the Core Research for Evolutional Science and Technology of Japan Science and Technology Corporation, by a Grant-in-Aid for Scientific Research (B) and Creative Basic Research of the Ministry of Education, Science, Sports and Culture, by the Ohara Foundation for Agricultural Sciences, and by the Joint Research Project under the Japan-Korea Basic Scientific Cooperation Program of Japan Society for Promotion of Science.

* Corresponding author; e-mail bezaki{at}rib.okayama-u.ac.jp; fax 86-434-1249.

© 2000 American Society of Plant Physiologists


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