|
Plant Physiol, March 2000, Vol. 122, pp. 705-714
Expression of AtPRP3, a Proline-Rich Structural Cell Wall Protein
from Arabidopsis, Is Regulated by Cell-Type-Specific Developmental
Pathways Involved in Root Hair Formation1
Christine
Bernhardt and
Mary L.
Tierney*
Department of Botany and Agricultural Biochemistry, University of
Vermont, Burlington, Vermont 05405
The tightly regulated expression
patterns of structural cell wall proteins in several plant species
indicate that they play a crucial role in determining the extracellular
matrix structure for specific cell types. We demonstrate that AtPRP3, a
proline-rich cell wall protein in Arabidopsis, is expressed in
root-hair-bearing epidermal cells at the root/shoot junction and within
the root differentiation zone of light-grown seedlings. Several lines
of evidence support a direct relationship between AtPRP3 expression and
root hair development. AtPRP3/ -glucuronidase (GUS) expression increased in roots of transgenic seedlings treated with either 1-aminocyclopropane-1-carboxylic acid (ACC) or
 -naphthaleneacetic acid (-NAA), compounds known to promote root
hair formation. In the presence of 1--(2-aminoethoxyvinyl)glycine
(AVG), an inhibitor of ethylene biosynthesis, AtPRP3/GUS expression was
strongly reduced, but could be rescued by co-addition of ACC or -NAA
to the growth medium. In addition, AtPRP3/GUS activity was enhanced in
ttg and gl2 mutant backgrounds that
exhibit ectopic root hairs, but was reduced in rhd6 and
35S-R root-hair-less mutant seedlings. These results
indicate that AtPRP3 is regulated by developmental pathways involved in
root hair formation, and are consistent with AtPRP3's contributing to
cell wall structure in Arabidopsis root hairs.
1
This research was supported by the U.S.
Department of Agriculture (grant no. NRICGP-95-02982). C.B. was
supported by experiment station grant no. 0171655.
*
Corresponding author; e-mail mtierney{at}zoo.uvm.edu; fax
802-656-0440.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S.-K. Won, Y.-J. Lee, H.-Y. Lee, Y.-K. Heo, M. Cho, and H.-T. Cho
cis-Element- and Transcriptome-Based Screening of Root Hair-Specific Genes and Their Functional Characterization in Arabidopsis
Plant Physiology,
July 1, 2009;
150(3):
1459 - 1473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Kim, S. H. Lee, S.-B. Choi, S.-K. Won, Y.-K. Heo, M. Cho, Y.-I. Park, and H.-T. Cho
Functional Conservation of a Root Hair Cell-Specific cis-Element in Angiosperms with Different Root Hair Distribution Patterns
PLANT CELL,
November 1, 2006;
18(11):
2958 - 2970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Xu and B. Scheres
Dissection of Arabidopsis ADP-RIBOSYLATION FACTOR 1 Function in Epidermal Cell Polarity
PLANT CELL,
February 1, 2005;
17(2):
525 - 536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Takahashi, K. Hirota, A. Kawahara, E. Hayakawa, and Y. Inoue
Randomization of Cortical Microtubules in Root Epidermal Cells Induces Root Hair Initiation in Lettuce (Lactuca sativa L.) Seedlings
Plant Cell Physiol.,
March 15, 2003;
44(3):
350 - 359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bucher, S. Brunner, P. Zimmermann, G. I. Zardi, N. Amrhein, L. Willmitzer, and J. W. Riesmeier
The Expression of an Extensin-Like Protein Correlates with Cellular Tip Growth in Tomato
Plant Physiology,
March 1, 2002;
128(3):
911 - 923.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Patterson
Cutting Loose. Abscission and Dehiscence in Arabidopsis
Plant Physiology,
June 1, 2001;
126(2):
494 - 500.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Murphy, K. R. Hoogner, W. A. Peer, and L. Taiz
Identification, Purification, and Molecular Cloning of N-1-Naphthylphthalmic Acid-Binding Plasma Membrane-Associated Aminopeptidases from Arabidopsis
Plant Physiology,
March 1, 2002;
128(3):
935 - 950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
