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Plant Physiol, March 2000, Vol. 122, pp. 767-774
Coordinate Changes in Carbon Partitioning and Plastidial
Metabolism during the Development of Oilseed
Rape Embryos1
Peter J.
Eastmond2 and
Stephen
Rawsthorne*
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH,
United Kingdom
Measurements
of metabolic fluxes in whole embryos and isolated plastids have
revealed major changes in the pathways of carbon utilization during
cotyledon filling by oilseed rape (Brassica napus L.)
embryos. In the early cotyledon stage (stage A), embryos used sucrose
(Suc) predominantly for starch synthesis. Plastids isolated from these
embryos imported glucose-6-phosphate (Glc-6-P) and partitioned it to
starch and fatty acids synthesis and to the oxidative pentose phosphate
pathway in the ratio of 2:1:1 on a hexose basis. Of the substrates
tested, Glc-6-P gave the highest rates of fatty acid synthesis by the
plastids and pyruvate was used weakly. By the mid- to late-cotyledon
stage (stage C), oil accumulation by the embryos was rapid, as was
their utilization of Suc for oil synthesis in vitro. Plastids from
C-stage embryos differed markedly from those of stage-A embryos: (a)
pyruvate uptake and utilization for fatty acid synthesis increased by
respectively 18- and 25-fold; (b) Glc-6-P partitioning was
predominantly to the oxidative pentose phosphate pathway (respective
ratios of 1:1:3); and (c) the rate of plastidial fatty acid synthesis
more than doubled. This increased rate of fatty synthesis was dependent upon the increase in pyruvate uptake and was mediated through the
induction of a saturable transporter activity.
1
This work was supported by the Biotechnology and
Biological Sciences Research Council through a Competitive Strategic
Grant to the John Innes Centre and a postgraduate studentship to
P.J.E.
2
Present address: Department of Biology,
University of York, P.O. Box 373, York YO10 5YW, UK.
*
Corresponding author; e-mail steve.rawsthorne{at}bbsrc.ac.uk; fax
44-1-603-259882/456844.
© 2000 American Society of Plant Physiologists
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