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Plant Physiol, March 2000, Vol. 122, pp. 775-782
Spatial Regulation of Pectic Polysaccharides in Relation to Pit
Fields in Cell Walls of Tomato Fruit Pericarp1
Caroline
Orfila and
J. Paul
Knox*
Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT,
United Kingdom
Scanning electron microscopic
examination of intact tomato (Lycopersicon esculentum)
pericarp and isolated pericarp cell walls revealed pit fields and
associated radiating ridges on the inner face of cell walls. In regions
of the cell wall away from pit fields, equivalent ridges occurred in
parallel arrays. Treatment of isolated cell walls with a calcium
chelator resulted in the loss of these ridges, indicating that they
contain homogalacturonan-rich pectic polysaccharides. Immunolabeling
procedures confirmed that pit fields and associated radiating ridges
contained homogalacturonan. Epitopes of the side chains of pectic
polysaccharides were not located in the same regions as
homogalacturonan and were spatially regulated in relation to pit
fields. A (1 4)- -galactan epitope was absent from cell walls in
regions of pit fields. A (15)- -arabinan epitope occurred most
abundantly at the inner face of cell walls in regions surrounding the
pit fields.
1
C.O. was supported by a U.K. Biotechnology and
Biological Science Research Council CASE studentship with
Horticulture Research International.
*
Corresponding author; e-mail j.p.knox{at}leeds.ac.uk; fax
44-113-2333144.
© 2000 American Society of Plant Physiologists
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