|
Plant Physiol, March 2000, Vol. 122, pp. 783-792
Isolation and Characterization of HvNRT2.3
and HvNRT2.4, cDNAs Encoding High-Affinity
Nitrate Transporters from Roots of Barley1
Joseph John
Vidmar,
Degen
Zhuo,
M. Yaeesh
Siddiqi, and
Anthony D.M.
Glass*
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z4
Two full-length cDNAs,
HvNRT2.3 and HvNRT2.4, were isolated from
roots of barley (Hordeum vulgare), using reverse
transcriptase-PCR and RACE-PCR. The corresponding polypeptides,
consisting of 507 amino acids (molecular masses of 54.6 kD), belong to
the major facilitator superfamily (MFS), and are closely related
(>87% identity) to those encoded by HvNRT2.1 and
HvNRT2.2 (formerly BCH1 and
BCH2, respectively) from roots of barley. The latter are
considered to encode inducible high-affinity
NO3 transporters (Trueman et al., 1996).
HvNRT2 transcripts were undetectable in
NO3-deprived plants. Following exposure to
either NO3 or NO2,
transcript abundance and 13NO3
influx increased to a maximum by 6 to 12 h, then declined in HvNRT2.1, HvNRT2.2, and
HvNRT2.3. The pattern of HvNRT2.4
transcript abundance was different, remaining high after achieving peak
abundance. When external NO3 concentrations
were varied from 0 to 500 µM under steady-state conditions of NO3 supply,
HvNRT2 transcript accumulation and
13NO3 influx were highest in 50 µM NO3 -grown
plants. When NH4+ was provided
together with NO3, transcript accumulation
during the first 2 h was similar to that due to
NO3 alone, but by 4 h the transcript
level was significantly reduced. HvNRT2 transcript was
undetectable in leaf tissues.
1
This work was supported by Natural Sciences and
Engineering Research Council of Canada Strategic and Research grants to
A.D.M.G.
*
Corresponding author; e-mail aglass{at}unixg.ubc.ca; fax
604-822-6089.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
A. Camargo, A. Llamas, R. A. Schnell, J. J. Higuera, D. Gonzalez-Ballester, P. A. Lefebvre, E. Fernandez, and A. Galvan
Nitrate Signaling by the Regulatory Gene NIT2 in Chlamydomonas
PLANT CELL,
November 1, 2007;
19(11):
3491 - 3503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Hirel, J. Le Gouis, B. Ney, and A. Gallais
The challenge of improving nitrogen use efficiency in crop plants: towards a more central role for genetic variability and quantitative genetics within integrated approaches
J. Exp. Bot.,
July 1, 2007;
58(9):
2369 - 2387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tsujimoto, H. Yamazaki, S.-i. Maeda, and T. Omata
Distinct Roles of Nitrate and Nitrite in Regulation of Expression of the Nitrate Transport Genes in the Moss Physcomitrella patens
Plant Cell Physiol.,
March 1, 2007;
48(3):
484 - 497.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Li, Y. Wang, M. Okamoto, N. M. Crawford, M. Y. Siddiqi, and A. D.M. Glass
Dissection of the AtNRT2.1:AtNRT2.2 Inducible High-Affinity Nitrate Transporter Gene Cluster
Plant Physiology,
January 1, 2007;
143(1):
425 - 433.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Santi, G. Locci, R. Monte, R. Pinton, and Z. Varanini
Induction of nitrate uptake in maize roots: expression of a putative high-affinity nitrate transporter and plasma membrane H+-ATPase isoforms
J. Exp. Bot.,
August 1, 2003;
54(389):
1851 - 1864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Okamoto, J. J. Vidmar, and A. D. M. Glass
Regulation of NRT1 and NRT2 Gene Families of Arabidopsis thaliana: Responses to Nitrate Provision
Plant Cell Physiol.,
March 15, 2003;
44(3):
304 - 317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Quaggiotti, B. Ruperti, P. Borsa, T. Destro, and M. Malagoli
Expression of a putative high-affinity NO3- transporter and of an H+-ATPase in relation to whole plant nitrate transport physiology in two maize genotypes differently responsive to low nitrogen availability
J. Exp. Bot.,
March 1, 2003;
54(384):
1023 - 1031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Orsel, S. Filleur, V. Fraisier, and F. Daniel-Vedele
Nitrate transport in plants: which gene and which control?
J. Exp. Bot.,
April 15, 2002;
53(370):
825 - 833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Cerezo, P. Tillard, S. Filleur, S. Munos, F. Daniel-Vedele, and A. Gojon
Major Alterations of the Regulation of Root NO3{-} Uptake Are Associated with the Mutation of Nrt2.1 and Nrt2.2 Genes in Arabidopsis
Plant Physiology,
September 1, 2001;
127(1):
262 - 271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Wang, D. F. Garvin, and L. V. Kochian
Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals Novel Genes That May Play a Role in Nitrogen Nutrition
Plant Physiology,
September 1, 2001;
127(1):
345 - 359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Aslam, R. L. Travis, and D. W. Rains
Inhibition of Net Nitrate Uptake by Ammonium in Pima and Acala Cotton Roots
Crop Sci.,
July 1, 2001;
41(4):
1130 - 1136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Vidmar, D. Zhuo, M. Y. Siddiqi, J. K. Schjoerring, B. Touraine, and A. D.M. Glass
Regulation of High-Affinity Nitrate Transporter Genes and High-Affinity Nitrate Influx by Nitrogen Pools in Roots of Barley
Plant Physiology,
May 1, 2000;
123(1):
307 - 318.
[Abstract]
[Full Text]
|
|
|
|
|
