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Plant Physiol, March 2000, Vol. 122, pp. 823-834

A Weakly Voltage-Dependent, Nonselective Cation Channel Mediates Toxic Sodium Influx in Wheat1

Romola Jane Davenport* and Mark Tester

Department of Plant Sciences, Downing Street, Cambridge CB2 3EA, United Kingdom

To determine the transporters responsible for toxic Na+ influx in wheat (Triticum aestivum), root plasma membrane preparations were screened using the planar lipid bilayer technique as an assay for Na+-permeable ion channel activity. The predominant channel in the bilayer was a 44-pS channel that we called the nonselective cation (NSC) channel, which was nonselective for monovalent cations and weakly voltage dependent. Single channel characteristics of the NSC channel were compared with 22Na+ influx into excised root segments. Na+ influx through the NSC channel resembled 22Na+ influx in its partial sensitivity to inhibition by Ca2+, Mg2+, and Gd3+, and its insensitivity to all other inhibitors tested (tetraethylammonium, quinine, Cs+, tetrodotoxin, verapamil, amiloride, and flufenamate). Na+ influx through the NSC channel also closely resembled an instantaneous current in wheat root protoplasts (S.D. Tyerman, M. Skerrett, A. Garill, G.P. Findlay, R. Leigh [1997] J Exp Bot 48: 459-480) in its permeability sequence, selectivity for K+ over Na+ (approximately 1.25), insensitivity to tetraethylammonium, voltage independence, and partial sensitivity to Ca2+. Comparison of tissue, protoplast (S.D. Tyerman, M. Skerrett, A. Garill, G.P. Findlay, R. Leigh [1997] J Exp Bot 48: 459-480), and single- channel data indicate that toxic Na+ influx is catalyzed by a single transporter, and this is likely to be the NSC channel identified in planar lipid bilayers.

1 This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (to M.T.) and a U.K. Commonwealth scholarship (to R.J.D.).

* Corresponding author; e-mail rjd23{at}cus.cam.ac.uk; fax 44-1223-333953.

© 2000 American Society of Plant Physiologists


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