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Plant Physiol, March 2000, Vol. 122, pp. 845-852
Import of Lyso-Phosphatidylcholine into Chloroplasts Likely at
the Origin of Eukaryotic Plastidial Lipids1
Sébastien
Mongrand,2
Claude
Cassagne, and
Jean-Jacques
Bessoule*
Unité Mixte de Recherche 5544, Centre National de la
Recherche Scientifique (S.M., C.C., J.-J.B.), and Ecole
Supérieure de Technologie des Biomolécules de Bordeaux
(C.C.), Université Victor Segalen Bordeaux 2, 146, rue Léo
Saignat-Case 92, 33076 Bordeaux cédex, France
Plastids
rely on the import of extraplastidial precursor for the synthesis of
their own lipids. This key phenomenon in the formation of plastidial
phosphatidylcholine (PC) and of the most abundant lipids on earth,
namely galactolipids, is poorly understood. Various suggestions have
been made on the nature of the precursor molecule(s) transferred to
plastids, but despite general agreement that PC or a close metabolite
plays a central role, there is no clear-cut answer to this question
because of a lack of conclusive experimental data. We therefore
designed experiments to discriminate between a transfer of PC,
1-acylglycero phosphorylcholine (lyso-PC), or glycerophosphorylcholine.
After pulse-chase experiments with glycerol and acetate, plastids of
leek (Allium porrum L.) seedlings were purified. The
labels of the glycerol moiety and the sn-1- and
sn-2-bound fatty acids of plastidial lipids were
determined and compared with those associated with the extraplastidial
PC. After import, plastid lipids contained the glycerol moiety and the
fatty acids esterified to the sn-1 position originating
from the extraplastidial PC; no import of sn-2-bound
fatty acid was detected. These results rule out a transfer of PC or
glycerophosphorylcholine, and are totally explained by an import of
lyso-PC molecules used subsequently as precursor for the synthesis of
eukaryotic plastid lipids.
1
This work was in part supported by the Conseil
Régional d'Aquitaine (France). S.M. was supported by a grant
from the Ministère de l'Education Nationale de la Recherche et
des Technologies.
2
Present address: Laboratory of Plant Molecular
Biology, The Rockefeller University, 1230 York Avenue, New York, NY
10021-6399.
*
Corresponding author; e-mail
Jean-Jacques.Bessoule{at}biomemb.u-bordeaux2.fr; fax
1-33-0-556-835-161.
© 2000 American Society of Plant Physiologists
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