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Plant Physiol, March 2000, Vol. 122, pp. 933-944
Expression of Tryptophan Decarboxylase and Tyrosine Decarboxylase
Genes in Tobacco Results in Altered Biochemical and Physiological
Phenotypes1
Gabriel
Guillet,
Julie
Poupart,
Juan
Basurco, and
Vincenzo
De Luca*
Department of Biological Sciences, University of Montréal,
Montréal, Québec, Canada H3C 3J7
The substrate specificity of
tryptophan (Trp) decarboxylase (TDC) for Trp and tyrosine (Tyr)
decarboxylase (TYDC) for Tyr was used to modify the in vivo pools of
these amino acids in transgenic tobacco. Expression of TDC and TYDC was
shown to deplete the levels of Trp and Tyr, respectively, during
seedling development. The creation of artificial metabolic sinks for
Trp and Tyr also drastically affected the levels of phenylalanine, as
well as those of the non-aromatic amino acids methionine, valine, and
leucine. Transgenic seedlings also displayed a root-curling phenotype
that directly correlated with the depletion of the Trp pool.
Non-transformed control seedlings could be induced to display this
phenotype after treatment with inhibitors of auxin translocation such
as 2,3,5-triiodobenzoic acid or N-1-naphthylphthalamic
acid. The depletion of aromatic amino acids was also correlated with
increases in the activities of the shikimate and phenylpropanoid
pathways in older, light-treated transgenic seedlings expressing TDC,
TYDC, or both. These results provide in vivo confirmation that aromatic
amino acids exert regulatory feedback control over carbon flux through
the shikimate pathway, as well as affecting pathways outside of
aromatic amino acid biosynthesis.
1
This work was supported by a research grant (to
V.D.L.) and a postdoctoral grant (to G.G.) from the Natural Sciences
and Engineering Research Council of Canada.
*
Corresponding author; e-mail vince.deluca{at}nabri.novartis.com;
fax 919-541-8610.
© 2000 American Society of Plant Physiologists
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