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Plant Physiol, March 2000, Vol. 122, pp. 977-984
Characterization of Ripening-Regulated cDNAs and Their Expression
in Ethylene-Suppressed Charentais Melon Fruit1
Kristen A.
Hadfield,2
Tam
Dang,
Monique
Guis,3
Jean-Claude
Pech,
Mondher
Bouzayen, and
Alan B.
Bennett*
Mann Laboratory, Department of Vegetable Crops, University of
California, Davis, California 95616 (K.A.H., T.D., M.G., A.B.B.);
and Ecole Nationale Supérieure Agronomique d' Toulouse, Avenue
de l'Agrobiopole, B.P. 107, Auzeville Tolosan, 31326 Castanet Tolosan
cedex, France (J.-C.P., M.B.)
Charentais
melons (Cucumis melo cv Reticulatus) are climacteric and
undergo extremely rapid ripening. Sixteen cDNAs corresponding to mRNAs
whose abundance is ripening regulated were isolated to characterize the
changes in gene expression that accompany this very rapid ripening
process. Sequence comparisons indicated that eight of these cDNA clones
encoded proteins that have been previously characterized, with one
corresponding to ACC (1-aminocyclopropane-1-carboxylic acid) oxidase,
three to proteins associated with pathogen responses, two to proteins
involved in sulfur amino acid biosynthesis, and two having significant
homology to a seed storage protein or a yeast secretory protein. The
remaining eight cDNA sequences did not reveal significant sequence
similarities to previously characterized proteins. The majority of the
16 ripening-regulated cDNAs corresponded to mRNAs that were fruit
specific, although three were expressed at low levels in vegetative
tissues. When examined in transgenic antisense ACC oxidase melon fruit,
three distinct patterns of mRNA accumulation were observed. One group
of cDNAs corresponded to mRNAs whose abundance was reduced in
transgenic fruit but inducible by ethylene treatment, indicating that
these genes are directly regulated by ethylene. A second group of mRNAs
was not significantly altered in the transgenic fruit and was
unaffected by treatment with ethylene, indicating that these genes are
regulated by ethylene-independent developmental cues. The third and
largest group of cDNAs showed an unexpected pattern of expression, with
levels of mRNA reduced in transgenic fruit and remaining low after
exposure to ethylene. Regulation of this third group of genes thus
appears to ethylene independent, but may be regulated by developmental
cues that require ethylene at a certain stage in fruit development. The
results confirm that both ethylene-dependent and ethylene-independent pathways of gene regulation coexist in climacteric fruit.
1
This research was supported by grants to A.B.B.
from Zeneca Plant Science and by the U.S. Department of
Agriculture-National Research Initiative (grant no. 97-35304-4627).
2
Present address: Monsanto Co., 1920 Fifth Street, Davis,
CA 95616.
3
Present address: RhoBio, 2 rue Gaston Crémieux,
B.P. 210, 91007 Evry-cedex, France.
*
Corresponding author; e-mail abbennett{at}ucdavis.edu; fax
530-752-4554.
© 2000 American Society of Plant Physiologists
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