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Plant Physiol, April 2000, Vol. 122, pp. 1003-1014

Activation Tagging in Arabidopsis1

Detlef Weigel,* Ji Hoon Ahn,2 Miguel A. Blázquez,2 Justin O. Borevitz,2 Sioux K. Christensen,2 Christian Fankhauser,2 Cristina Ferrándiz,2 Igor Kardailsky,23 Elizabeth J. Malancharuvil,2 Michael M. Neff,24 Jasmine Thuy Nguyen,25 Shusei Sato,2 Zhi-Yong Wang,2 Yiji Xia,2 Richard A. Dixon, Maria J. Harrison, Chris J. Lamb,6 Martin F. Yanofsky, and Joanne Chory

Plant Biology Laboratory (D.W., J.H.A., M.A.B., J.O.B., S.K.C., C.Fankhauser, I.K., E.J.M., M.M.N., J.T.N., Z.-Y.W., Y.X., C.J.L., J.C.) and Howard Hughes Medical Institute (J.C.), The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037; Department of Biology, University of California at San Diego, La Jolla, California 92093-0116 (C.Ferrándiz, S.S., M.F.Y.); and Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, Oklahoma 73402 (I.K., Y.X., R.A.D., M.J.H.)

Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant mutants with various phenotypes have been isolated. Analysis of a subset of mutants has shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to 3.6 kb. In at least one case, the CaMV 35S enhancers led primarily to an enhancement of the endogenous expression pattern rather than to constitutive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the complete CaMV 35S promoter. This has important implications for the spectrum of genes that will be discovered by this method.


1 This work was supported by grants from the National Science Foundation (no. MCB-9723823 to D.W., no. IBN-9728402 to M.F.Y., and no. MCB-9631390 to J.C.), the National Institutes of Health (no. R01 GM52413 to J.C.), and by the Samuel Roberts Noble Foundation. This work was also supported by a Research Experience for Undergraduates Supplement to National Science Foundation grant (no. IBN-9406948 to D.W.), and fellowships from the Korea Science and Engineering Foundation and the Hoffman Foundation (J.H.A.), the Spanish Ministry of Education (M.A.B., C.Ferrándiz), the National Science Foundation (S.K.C., Z.-Y.W.), the Human Frontiers Science Program Organization (M.A.B., C.Fa.), the Swiss National Science Foundation (C.Fankhauser), the National Institutes of Health (M.M.N.), and the Kazusa DNA Research Foundation (S.S.). D.W. was a National Science Foundation Young Investigator and J.C. was an Associate Investigator of the Howard Hughes Medical Institute.

2 These authors contributed equally to this study and are listed alphabetically.

3 Present address: Plant Gene Expression Center, 800 Buchanan Street, Albany, CA 94710.

4 Present address: Department of Biology, Washington University, One Brookings Drive, St. Louis, MO 63130.

5 Present address: Akkadix Corporation, 11099 North Torrey Pines Road, La Jolla, CA 92037.

6 Present address: John Innes Centre, Colney Lane, Norwich NR4 7UH, UK.

* Corresponding author; e-mail weigel{at}salk.edu; fax 858-558-6379.

© 2000 American Society of Plant Physiologists



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Plant CellHome page
J. M. Ward, A. M. Smith, P. K. Shah, S. E. Galanti, H. Yi, A. J. Demianski, E. van der Graaff, B. Keller, and M. M. Neff
A New Role for the Arabidopsis AP2 Transcription Factor, LEAFY PETIOLE, in Gibberellin-Induced Germination Is Revealed by the Misexpression of a Homologous Gene, SOB2/DRN-LIKE
PLANT CELL, January 1, 2006; 18(1): 29 - 39.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
J. M. Kuhn, A. Boisson-Dernier, M. B. Dizon, M. H. Maktabi, and J. I. Schroeder
The Protein Phosphatase AtPP2CA Negatively Regulates Abscisic Acid Signal Transduction in Arabidopsis, and Effects of abh1 on AtPP2CA mRNA
Plant Physiology, January 1, 2006; 140(1): 127 - 139.
[Abstract] [Full Text] [PDF]


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Genes Dev.Home page
M. K. Jakobsen,, L. R. Poulsen,, A. Schulz,, P. Fleurat-Lessard,, A. Moller,, S. Husted,, M. Schiott,, A. Amtmann,, and M. G. Palmgren,
Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a Type V P-type ATPase
Genes & Dev., November 15, 2005; 19(22): 2757 - 2769.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
G. Qin, H. Gu, Y. Zhao, Z. Ma, G. Shi, Y. Yang, E. Pichersky, H. Chen, M. Liu, Z. Chen, et al.
An Indole-3-Acetic Acid Carboxyl Methyltransferase Regulates Arabidopsis Leaf Development
PLANT CELL, October 1, 2005; 17(10): 2693 - 2704.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
X. Chen, S. M. Goodwin, X. Liu, X. Chen, R. A. Bressan, and M. A. Jenks
Mutation of the RESURRECTION1 Locus of Arabidopsis Reveals an Association of Cuticular Wax with Embryo Development
Plant Physiology, October 1, 2005; 139(2): 909 - 919.
[Abstract] [Full Text] [PDF]


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ANN BOT (LOND)Home page
R. PIERIK, F. F. MILLENAAR, A. J. M. PEETERS, and L. A. C. J. VOESENEK
New Perspectives in Flooding Research: the Use of Shade Avoidance and Arabidopsis thaliana
Ann. Bot., September 1, 2005; 96(4): 533 - 540.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
C. Woodward, S. M. Bemis, E. J. Hill, S. Sawa, T. Koshiba, and K. U. Torii
Interaction of Auxin and ERECTA in Elaborating Arabidopsis Inflorescence Architecture Revealed by the Activation Tagging of a New Member of the YUCCA Family Putative Flavin Monooxygenases
Plant Physiology, September 1, 2005; 139(1): 192 - 203.
[Abstract] [Full Text] [PDF]


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Nucleic Acids ResHome page
G. Tan, Y. Gao, M. Shi, X. Zhang, S. He, Z. Chen, and C. An
SiteFinding-PCR: a simple and efficient PCR method for chromosome walking
Nucleic Acids Res., August 2, 2005; 33(13): e122 - e122.
[Abstract] [Full Text] [PDF]


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J Exp BotHome page
L. Salaita, R. K. Kar, M. Majee, and A. B. Downie
Identification and characterization of mutants capable of rapid seed germination at 10 {degrees}C from activation-tagged lines of Arabidopsis thaliana
J. Exp. Bot., August 1, 2005; 56(418): 2059 - 2069.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
R. Lin and H. Wang
Two Homologous ATP-Binding Cassette Transporter Proteins, AtMDR1 and AtPGP1, Regulate Arabidopsis Photomorphogenesis and Root Development by Mediating Polar Auxin Transport
Plant Physiology, June 1, 2005; 138(2): 949 - 964.
[Abstract] [Full Text] [PDF]


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DevelopmentHome page
M. Norberg, M. Holmlund, and O. Nilsson
The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Development, May 1, 2005; 132(9): 2203 - 2213.
[Abstract] [Full Text] [PDF]


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JCBHome page
Y. Gu, Y. Fu, P. Dowd, S. Li, V. Vernoud, S. Gilroy, and Z. Yang
A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes
J. Cell Biol., April 11, 2005; 169(1): 127 - 138.
[Abstract] [Full Text] [PDF]


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Plant Cell PhysiolHome page
T. Masaki, N. Mitsui, H. Tsukagoshi, T. Nishii, A. Morikami, and K. Nakamura
ACTIVATOR of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana Transactivates Sugar-inducible Promoters
Plant Cell Physiol., April 1, 2005; 46(4): 547 - 556.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
J. M. Ward, C. A. Cufr, M. A. Denzel, and M. M. Neff
The Dof Transcription Factor OBP3 Modulates Phytochrome and Cryptochrome Signaling in Arabidopsis
PLANT CELL, February 1, 2005; 17(2): 475 - 485.
[Abstract] [Full Text] [PDF]


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J. Biol. Chem.Home page
J. E. Turner, K. Greville, E. C. Murphy, and M. A. Hooks
Characterization of Arabidopsis Fluoroacetate-resistant Mutants Reveals the Principal Mechanism of Acetate Activation for Entry into the Glyoxylate Cycle
J. Biol. Chem., January 28, 2005; 280(4): 2780 - 2787.
[Abstract] [Full Text] [PDF]


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Plant Cell PhysiolHome page
G. An, S. Lee, S.-H. Kim, and S.-R. Kim
Molecular Genetics Using T-DNA in Rice
Plant Cell Physiol., January 15, 2005; 46(1): 14 - 22.
[Abstract] [Full Text] [PDF]


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Genes Dev.Home page
A. Falciatore, L. Merendino, F. Barneche, M. Ceol, R. Meskauskiene, K. Apel, and J.-D. Rochaix
The FLP proteins act as regulators of chlorophyll synthesis in response to light and plastid signals in Chlamydomonas
Genes & Dev., January 1, 2005; 19(1): 176 - 187.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
M. J. Prigge, D. Otsuga, J. M. Alonso, J. R. Ecker, G. N. Drews, and S. E. Clark
Class III Homeodomain-Leucine Zipper Gene Family Members Have Overlapping, Antagonistic, and Distinct Roles in Arabidopsis Development
PLANT CELL, January 1, 2005; 17(1): 61 - 76.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
S. D. Michaels, E. Himelblau, S. Y. Kim, F. M. Schomburg, and R. M. Amasino
Integration of Flowering Signals in Winter-Annual Arabidopsis
Plant Physiology, January 1, 2005; 137(1): 149 - 156.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
S. Ren, J. S. Johnston, D. E. Shippen, and T. D. McKnight
TELOMERASE ACTIVATOR1 Induces Telomerase Activity and Potentiates Responses to Auxin in Arabidopsis
PLANT CELL, November 1, 2004; 16(11): 2910 - 2922.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
Y. Zhao, L. Medrano, K. Ohashi, J. C. Fletcher, H. Yu, H. Sakai, and E. M. Meyerowitz
HANABA TARANU Is a GATA Transcription Factor That Regulates Shoot Apical Meristem and Flower Development in Arabidopsis
PLANT CELL, October 1, 2004; 16(10): 2586 - 2600.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
H. Muto, N. Yabe, T. Asami, K. Hasunuma, and K. T. Yamamoto
Overexpression of Constitutive Differential Growth 1 Gene, Which Encodes a RLCKVII-Subfamily Protein Kinase, Causes Abnormal Differential and Elongation Growth after Organ Differentiation in Arabidopsis
Plant Physiology, October 1, 2004; 136(2): 3124 - 3133.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
D. E. Salt
Update on Plant Ionomics
Plant Physiology, September 1, 2004; 136(1): 2451 - 2456.
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Plant CellHome page
G. Pilot, H. Stransky, D. F. Bushey, R. Pratelli, U. Ludewig, V. P.M. Wingate, and W. B. Frommer
Overexpression of GLUTAMINE DUMPER1 Leads to Hypersecretion of Glutamine from Hydathodes of Arabidopsis Leaves
PLANT CELL, July 1, 2004; 16(7): 1827 - 1840.
[Abstract] [Full Text] [PDF]


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Mol. Biol. CellHome page
Y. Ivanova, M. D. Smith, K. Chen, and D. J. Schnell
Members of the Toc159 Import Receptor Family Represent Distinct Pathways for Protein Targeting to Plastids
Mol. Biol. Cell, July 1, 2004; 15(7): 3379 - 3392.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
G. Inan, Q. Zhang, P. Li, Z. Wang, Z. Cao, H. Zhang, C. Zhang, T. M. Quist, S. M. Goodwin, J. Zhu, et al.
Salt Cress. A Halophyte and Cryophyte Arabidopsis Relative Model System and Its Applicability to Molecular Genetic Analyses of Growth and Development of Extremophiles
Plant Physiology, July 1, 2004; 135(3): 1718 - 1737.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
J. C. Sedbrook, D. W. Ehrhardt, S. E. Fisher, W.-R. Scheible, and C. R. Somerville
The Arabidopsis SKU6/SPIRAL1 Gene Encodes a Plus End-Localized Microtubule-Interacting Protein Involved in Directional Cell Expansion
PLANT CELL, June 1, 2004; 16(6): 1506 - 1520.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
J. Molinier, C. Ramos, O. Fritsch, and B. Hohn
CENTRIN2 Modulates Homologous Recombination and Nucleotide Excision Repair in Arabidopsis
PLANT CELL, June 1, 2004; 16(6): 1633 - 1643.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
W.-Y. Song, E. Martinoia, J. Lee, D. Kim, D.-Y. Kim, E. Vogt, D. Shim, K. S. Choi, I. Hwang, and Y. Lee
A Novel Family of Cys-Rich Membrane Proteins Mediates Cadmium Resistance in Arabidopsis
Plant Physiology, June 1, 2004; 135(2): 1027 - 1039.
[Abstract] [Full Text] [PDF]


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Proc. Natl. Acad. Sci. USAHome page
Y. Choi, J. J. Harada, R. B. Goldberg, and R. L. Fischer
An invariant aspartic acid in the DNA glycosylase domain of DEMETER is necessary for transcriptional activation of the imprinted MEDEA gene
PNAS, May 11, 2004; 101(19): 7481 - 7486.
[Abstract] [Full Text] [PDF]


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Plant Physiol.Home page
G.-W. Tian, A. Mohanty, S. N. Chary, S. Li, B. Paap, G. Drakakaki, C. D. Kopec, J. Li, D. Ehrhardt, D. Jackson, et al.
High-Throughput Fluorescent Tagging of Full-Length Arabidopsis Gene Products in Planta
Plant Physiology, May 1, 2004; 135(1): 25 - 38.
[Abstract] [Full Text] [PDF]


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DevelopmentHome page
J. R. Dinneny, R. Yadegari, R. L. Fischer, M. F. Yanofsky, and D. Weigel
The role of JAGGED in shaping lateral organs
Development, March 1, 2004; 131(5): 1101 - 1110.
[Abstract] [Full Text] [PDF]


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Plant CellHome page
M.-H. Lim, J. Kim, Y.-S. Kim, K.-S. Chung, Y.-H. Seo, I. Lee, J. Kim, C. B. Hong, H.-J. Kim, and C.-M. Park
A New Arabidopsis Gene, FLK, Encodes an RNA Binding Protein with K Homology Motifs and Regulates Flowering Time via FLOWERING LOCUS C
PLANT CELL, March 1, 2004; 16(3): 731 - 740.
[Abstract] [Full Text] [PDF]


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Genes Dev.Home page
S. Mora-Garcia, G. Vert, Y. Yin, A. Cano-Delgado, H. Cheong, and J. Chory
Nuclear protein phosphatases with Kelch-repeat domains modulate the response to brassinosteroids in Arabidopsis
Genes & Dev., February 15, 2004; 18(4): 448 - 460.
[Abstract] [Full Text] [PDF]




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