|
Plant Physiol, April 2000, Vol. 122, pp. 1035-1044
Spatio-Temporal Accumulation and Activity of Calcium-Dependent
Protein Kinases during Embryogenesis, Seed Development, and Germination
in Sandalwood1
Veena S.
Anil,
Alice C.
Harmon, and
K. Sankara
Rao*
Department of Biochemistry, Indian Institute of Science,
Bangalore-560 012, India (V.S.A., K.S.R.); and Department of Botany,
University of Florida, Gainesville, Florida 32611-8526 (A.C.H.)
Western-blot analysis and protein
kinase assays identified two Ca2+-dependent protein kinases
(CDPKs) of 55 to 60 kD in soluble protein extracts of embryogenic
cultures of sandalwood (Santalum album L.). However,
these sandalwood CDPKs (swCDPKs) were absent in plantlets regenerated
from somatic embryos. swCDPKs exhibited differential expression
(monitored at the level of the protein) and activity in different
developmental stages. Zygotic embryos, seedlings, and endosperm showed
high accumulation of swCDPK, but the enzyme was not detected in the
soluble proteins of shoots and flowers. swCDPK exhibited a temporal
pattern of expression in endosperm, showing high accumulation and
activity in mature fruit and germinating stages; the enzyme was
localized strongly in the storage bodies of the endosperm cells. The
study also reports for the first time to our knowledge a
post-translational inhibition/inactivation of swCDPK in zygotic embryos
during seed dormancy and early stages of germination. The temporal
expression of swCDPK during somatic/zygotic embryogenesis, seed
maturation, and germination suggests involvement of the enzyme in these
developmental processes.
1
This research was supported by a grant from the
Department of Science and Technology, Government of India. Experiments
were carried out at the Department of Biochemistry, Indian Institute of
Science, Bangalore-560 012, India. Confocal microscopy was carried out
in the facility supported by the Biotechnology Department, Government
of India.
*
Corresponding author; e-mail baradwaj{at}biochem.iisc.ernet.in; fax
91-80-3341814 or 91-80-3341683.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
X. Pang, T. Halaly, O. Crane, T. Keilin, A. Keren-Keiserman, A. Ogrodovitch, D. Galbraith, and E. Or
Involvement of calcium signalling in dormancy release of grape buds
J. Exp. Bot.,
September 1, 2007;
58(12):
3249 - 3262.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Raichaudhuri, R. Bhattacharyya, S. Chaudhuri, P. Chakrabarti, and M. DasGupta
Domain Analysis of a Groundnut Calcium-dependent Protein Kinase: NUCLEAR LOCALIZATION SEQUENCE IN THE JUNCTION DOMAIN IS COUPLED WITH NONCONSENSUS CALCIUM BINDING DOMAINS
J. Biol. Chem.,
April 14, 2006;
281(15):
10399 - 10409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Ludwig, T. Romeis, and J. D. G. Jones
CDPK-mediated signalling pathways: specificity and cross-talk
J. Exp. Bot.,
January 2, 2004;
55(395):
181 - 188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dammann, A. Ichida, B. Hong, S. M. Romanowsky, E. M. Hrabak, A. C. Harmon, B. G. Pickard, and J. F. Harper
Subcellular Targeting of Nine Calcium-Dependent Protein Kinase Isoforms from Arabidopsis
Plant Physiology,
August 1, 2003;
132(4):
1840 - 1848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. S. Anil, A. C. Harmon, and K. S. Rao
Temporal Association of Ca2+-Dependent Protein Kinase with Oil Bodies during Seed Development in Santalum album L.: Its Biochemical Characterization and Significance
Plant Cell Physiol.,
April 15, 2003;
44(4):
367 - 376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Chang, M. H. Cho, S.-K. Kim, J. S. Lee, A. Kirakosyan, and P. B. Kaufman
Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots
J. Exp. Bot.,
March 1, 2003;
54(384):
1013 - 1022.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-H. Cheng, M. R. Willmann, H.-C. Chen, and J. Sheen
Calcium Signaling through Protein Kinases. The Arabidopsis Calcium-Dependent Protein Kinase Gene Family
Plant Physiology,
June 1, 2002;
129(2):
469 - 485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-C. Yoo, J.-Y. Lee, and W. J. Lucas
Analysis of the Complexity of Protein Kinases within the Phloem Sieve Tube System. CHARACTERIZATION OF CUCURBITA MAXIMA CALMODULIN-LIKE DOMAIN PROTEIN KINASE 1
J. Biol. Chem.,
May 3, 2002;
277(18):
15325 - 15332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. S. Anil and K. S. Rao
Calcium-Mediated Signaling during Sandalwood Somatic Embryogenesis. Role for Exogenous Calcium as Second Messenger
Plant Physiology,
August 1, 2000;
123(4):
1301 - 1312.
[Abstract]
[Full Text]
|
|
|
|
|
