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Plant Physiol, April 2000, Vol. 122, pp. 1089-1098
Gibberellins and Seed Development in Maize. II. Gibberellin
Synthesis Inhibition Enhances Abscisic Acid Signaling in Cultured
Embryos1
Constance N.
White2 and
Carol J.
Rivin*
Department of Botany and Plant Pathology, Center for Gene Research
and Biotechnology, Oregon State University, Corvallis, Oregon
97331-2902
Abscisic
acid (ABA) is required for seed maturation in maize (Zea
mays L.) and other plants. Gibberellins (GAs) are also present in developing maize embryos, and mutual antagonism of GAs and ABA
appears to govern the choice between precocious germination or
quiescence and maturation. Exogenous ABA can also induce quiescence and
maturation in immature maize embryos in culture. To examine the role of
GAs versus ABA in regulating maize embryo maturation, the effects of
modulating GA levels were compared with those of ABA in embryos
cultured at successive stages of development. The effects of GA
synthesis inhibition or exogenous GA application differed markedly in
embryos at different stages of development, indicating changes in both
endogenous GA levels and in the capacity for GA synthesis as
embryogenesis and maturation progress. In immature embryos, the
inhibition of GA synthesis mimicked the effects of exogenous ABA, as
shown by the suppression of germination, the acquisition of anthocyanin
pigments, and the accumulation of a variety of maturation-phase mRNAs.
We suggest that GA antagonizes ABA signaling in developing maize
embryos, and that the changing hormone balance provides temporal
control over the maturation phase.
1
This work was supported by the National Science
Foundation (grant nos. DCB9007481 and IBN-9318447 to C.J.R.). This is
Oregon Agricultural Experiment Station Technical Paper no. 11,607.
2
Present address: Linn Veterinary Hospital, 6011 Pacific Boulevard SW, Albany, OR 97321.
*
Corresponding author; e-mail rivinc{at}bcc.orst.edu; fax
541-737-3573.
© 2000 American Society of Plant Physiologists
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