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Plant Physiol, April 2000, Vol. 122, pp. 1119-1128

A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin1

Mónica Quiroga, Consuelo Guerrero, Miguel A. Botella, Araceli Barceló, Iraida Amaya, María I. Medina, Francisco J. Alonso, Silvia Milrad de Forchetti, Horacio Tigier, and Victoriano Valpuesta*

Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, 5800 Río Cuarto (Cba), Argentina (M.Q., M.I.M., S.M.d.F., H.T.); Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, 29071 Málaga, Spain (C.G., M.A.B., I.A., F.J.A., V.V.); and Centro de Investigación y Formación Agraria, Churriana, 29140 Málaga, Spain (A.B.)

The last step in the synthesis of lignin and suberin has been proposed to be catalyzed by peroxidases, although other proteins may also be involved. To determine which peroxidases are involved in the synthesis of lignin and suberin, five peroxidases from tomato (Lycopersicon esculentum) roots, representing the majority of the peroxidase activity in this organ, have been partially purified and characterized kinetically. The purified peroxidases with isoelectric point (pI) values of 3.6 and 9.6 showed the highest catalytic efficiency when the substrate used was syringaldazine, an analog of lignin monomer. Using a combination of transgenic expression and antibody recognition, we now show that the peroxidase pI 9.6 is probably encoded by TPX1, a tomato peroxidase gene we have previously isolated. In situ RNA hybridization revealed that TPX1 expression is restricted to cells undergoing synthesis of lignin and suberin. Salt stress has been reported to induce the synthesis of lignin and/or suberin. This stress applied to tomato caused changes in the expression pattern of TPX1 and induced the TPX1 protein. We propose that the TPX1 product is involved in the synthesis of lignin and suberin.

1 This research was supported by Comisión Interministerial de Ciencia y Tecnología (grant no. BIO94-0622-CO2-01) from the Ministerio de Educación y Ciencia, Spain, and by Consejo Investigaciones Científicas y Técnicas Provincia de Córdoba, Secretaría Ciencia y Técnica-Universidad Nacional Río Cuarto, and Consejo Nacional de Investigaciones Científicas y Técnicas from Argentina. M.Q. was the recipient of a fellowship from Consejo Investigaciones Científicas y Técnicas Provincia de Córdoba, Córdoba, Argentina, and she acknowledges the Agencia Esparíola de Cooperación Internacional, Spain, for short-term financial support to stay in Málaga, Spain.

* Corresponding author; e-mail valpuesta{at}uma.es; fax 34-95-213-1932.

© 2000 American Society of Plant Physiologists


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