Plant Physiol. Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (99)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quiroga, M.
Right arrow Articles by Valpuesta, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quiroga, M.
Right arrow Articles by Valpuesta, V.
Agricola
Right arrow Articles by Quiroga, M.
Right arrow Articles by Valpuesta, V.

Plant Physiol, April 2000, Vol. 122, pp. 1119-1128

A Tomato Peroxidase Involved in the Synthesis of Lignin and Suberin1

Mónica Quiroga, Consuelo Guerrero, Miguel A. Botella, Araceli Barceló, Iraida Amaya, María I. Medina, Francisco J. Alonso, Silvia Milrad de Forchetti, Horacio Tigier, and Victoriano Valpuesta*

Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, 5800 Río Cuarto (Cba), Argentina (M.Q., M.I.M., S.M.d.F., H.T.); Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, 29071 Málaga, Spain (C.G., M.A.B., I.A., F.J.A., V.V.); and Centro de Investigación y Formación Agraria, Churriana, 29140 Málaga, Spain (A.B.)

The last step in the synthesis of lignin and suberin has been proposed to be catalyzed by peroxidases, although other proteins may also be involved. To determine which peroxidases are involved in the synthesis of lignin and suberin, five peroxidases from tomato (Lycopersicon esculentum) roots, representing the majority of the peroxidase activity in this organ, have been partially purified and characterized kinetically. The purified peroxidases with isoelectric point (pI) values of 3.6 and 9.6 showed the highest catalytic efficiency when the substrate used was syringaldazine, an analog of lignin monomer. Using a combination of transgenic expression and antibody recognition, we now show that the peroxidase pI 9.6 is probably encoded by TPX1, a tomato peroxidase gene we have previously isolated. In situ RNA hybridization revealed that TPX1 expression is restricted to cells undergoing synthesis of lignin and suberin. Salt stress has been reported to induce the synthesis of lignin and/or suberin. This stress applied to tomato caused changes in the expression pattern of TPX1 and induced the TPX1 protein. We propose that the TPX1 product is involved in the synthesis of lignin and suberin.

1 This research was supported by Comisión Interministerial de Ciencia y Tecnología (grant no. BIO94-0622-CO2-01) from the Ministerio de Educación y Ciencia, Spain, and by Consejo Investigaciones Científicas y Técnicas Provincia de Córdoba, Secretaría Ciencia y Técnica-Universidad Nacional Río Cuarto, and Consejo Nacional de Investigaciones Científicas y Técnicas from Argentina. M.Q. was the recipient of a fellowship from Consejo Investigaciones Científicas y Técnicas Provincia de Córdoba, Córdoba, Argentina, and she acknowledges the Agencia Esparíola de Cooperación Internacional, Spain, for short-term financial support to stay in Málaga, Spain.

* Corresponding author; e-mail valpuesta{at}uma.es; fax 34-95-213-1932.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. A. Farag, B. E. Deavours, A. de Fatima, M. Naoumkina, R. A. Dixon, and L. W. Sumner
Integrated Metabolite and Transcript Profiling Identify a Biosynthetic Mechanism for Hispidol in Medicago truncatula Cell Cultures
Plant Physiology, November 1, 2009; 151(3): 1096 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 Tree PhysiolHome page
B. Aganchich, S. Wahbi, F. Loreto, and M. Centritto
Partial root zone drying: regulation of photosynthetic limitations and antioxidant enzymatic activities in young olive (Olea europaea) saplings
Tree Physiol, May 1, 2009; 29(5): 685 - 696.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Cosio and C. Dunand
Specific functions of individual class III peroxidase genes
J. Exp. Bot., February 1, 2009; 60(2): 391 - 408.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Marjamaa, E. M. Kukkola, and K. V. Fagerstedt
The role of xylem class III peroxidases in lignification
J. Exp. Bot., February 1, 2009; 60(2): 367 - 376.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Barel and I. Ginzberg
Potato skin proteome is enriched with plant defence components
J. Exp. Bot., September 1, 2008; 59(12): 3347 - 3357.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
Z. Hu, Q. Liu, M. Tan, H. Yi, and X. Deng
Lignin Deposition Converts Juice Sacs to "Brown Thorns" in a Citrus Triploid Hybrid
J. Amer. Soc. Hort. Sci., March 1, 2008; 133(2): 173 - 177.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. J. Reina, C. Guerrero, and A. Heredia
Isolation, characterization, and localization of AgaSGNH cDNA: a new SGNH-motif plant hydrolase specific to Agave americana L. leaf epidermis
J. Exp. Bot., August 1, 2007; 58(11): 2717 - 2731.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
A. H. L. A. N. Gunawardena, J. S. Greenwood, and N. G. Dengler
Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae)
Am. J. Botany, July 1, 2007; 94(7): 1116 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Soler, O. Serra, M. Molinas, G. Huguet, S. Fluch, and M. Figueras
A Genomic Approach to Suberin Biosynthesis and Cork Differentiation
Plant Physiology, May 1, 2007; 144(1): 419 - 431.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B.-R. Lee, K.-Y. Kim, W.-J. Jung, J.-C. Avice, A. Ourry, and T.-H. Kim
Peroxidases and lignification in relation to the intensity of water-deficit stress in white clover (Trifolium repens L.)
J. Exp. Bot., April 1, 2007; 58(6): 1271 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Ortega, S. C. Fry, and E. Taleisnik
Why are Chloris gayana leaves shorter in salt-affected plants? Analyses in the elongation zone
J. Exp. Bot., November 1, 2006; 57(14): 3945 - 3952.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Y. Sato, T. Demura, K. Yamawaki, Y. Inoue, S. Sato, M. Sugiyama, and H. Fukuda
Isolation and Characterization of a Novel Peroxidase Gene ZPO-C Whose Expression and Function are Closely Associated with Lignification during Tracheary Element Differentiation
Plant Cell Physiol., April 1, 2006; 47(4): 493 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Gabaldon, M. Lopez-Serrano, M. A. Pedreno, and A. R. Barcelo
Cloning and Molecular Characterization of the Basic Peroxidase Isoenzyme from Zinnia elegans, an Enzyme Involved in Lignin Biosynthesis
Plant Physiology, November 1, 2005; 139(3): 1138 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Sasaki, T. Iwai, S. Hiraga, K. Kuroda, S. Seo, I. Mitsuhara, A. Miyasaka, M. Iwano, H. Ito, H. Matsui, et al.
Ten Rice Peroxidases Redundantly Respond to Multiple Stresses Including Infection with Rice Blast Fungus
Plant Cell Physiol., October 15, 2004; 45(10): 1442 - 1452.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
N. FERNANDEZ-GARCIA, M. CARVAJAL, and E. OLMOS
Graft Union Formation in Tomato Plants: Peroxidase and Catalase Involvement
Ann. Bot., January 1, 2004; 93(1): 53 - 60.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Morohashi
Peroxidase activity develops in the micropylar endosperm of tomato seeds prior to radicle protrusion
J. Exp. Bot., July 1, 2002; 53(374): 1643 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
S. Hiraga, K. Sasaki, H. Ito, Y. Ohashi, and H. Matsui
A Large Family of Class III Plant Peroxidases
Plant Cell Physiol., May 1, 2001; 42(5): 462 - 468.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists