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Plant Physiol, April 2000, Vol. 122, pp. 1187-1192
The rug3 Locus of Pea Encodes Plastidial
Phosphoglucomutase1
Christopher J.
Harrison,
Ruth M.
Mould,
Mark J.
Leech,
Susan A.
Johnson,
Lynda
Turner,
Sabine L.
Schreck,
Kathleen M.
Baird,
Peter L.
Jack,2
Stephen
Rawsthorne,
Cliff L.
Hedley, and
Trevor L.
Wang*
John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United
Kingdom (C.J.H., M.J.L., S.A.J., L.T., S.L.S., S.R., C.L.H., T.L.W.);
Department of Plant Sciences, University of Cambridge, Cambridge CB2
3EA, United Kingdom (R.M.M.); Department of Genetics, University of
Cambridge, Cambridge CB2 3EH, United Kingdom (K.M.B.); and Plant
Breeding International Cambridge, Cambridge CB2 2LQ, United Kingdom
(P.L.J.)
Two cDNA clones were isolated from
pea (Pisum sativum L.) and their deduced amino acid
sequences shown to have significant homology to phosphoglucomutases
from eukaryotic and prokaryotic sources. The longer cDNA contained a
putative transit-peptide-encoding sequence, supporting the hypothesis
that the isolated clones represent the cytosolic and plastidial
isoforms of phosphoglucomutase in pea. Plastid protein import assays
confirmed that the putative plastidial isoform was targeted to the
plastid stroma where it was proteolytically processed. Expression,
co-segregation, linkage, and molecular analyses have confirmed that the
rug3 locus of pea encodes plastidial phosphoglucomutase.
Mutations at this locus result in a near-starchless phenotype of the plant.
1
The John Innes Centre is supported by a
grant-in-aid from the Biotechnology and Biological Sciences Research
Council. S.L.S. has a research studentship from the John Innes
Foundation and R.M.M. has a fellowship from the Royal Society.
2
Present address: Monsanto Agriculture, Maris Lane,
Cambridge CB2 2LQ, United Kingdom.
*
Corresponding author; e-mail trevor.wang{at}bbsrc.ac.uk; fax
44-1603-456844.
© 2000 American Society of Plant Physiologists
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