|
Plant Physiol, April 2000, Vol. 122, pp. 1201-1208
Inactivation of Photosystems I and II in Response to Osmotic
Stress in Synechococcus. Contribution of Water
Channels1
Suleyman I.
Allakhverdiev,
Atsushi
Sakamoto,
Yoshitaka
Nishiyama, and
Norio
Murata*
Department of Regulation Biology, National Institute for Basic
Biology, Okazaki 444-8585, Japan (S.I.A., A.S., Y.N., N.M.); and
Institute of Basic Biological Problems, Russian Academy of
Sciences, Pushchino, Moscow Region, 142292 Russia (S.I.A.)
The effects of osmotic stress due to
sorbitol on the photosynthetic machinery were investigated in the
cyanobacterium Synechococcus R-2. Incubation of cells in
1.0 M sorbitol inactivated photosystems I and II and
decreased the intracellular solute space by 50%. These effects of
sorbitol were reversible: Photosynthetic activity and cytoplasmic
volume returned to the original values after removal of the osmotic
stress. A blocker of water channels prevented the osmotic-stress-induced inactivation and shrinkage of the intracellular space. It also prevented the recovery of photosynthetic activity and
cytoplasmic volume when applied just before release from osmotic stress. Inhibition of protein synthesis by lincomycin had no
significant effects on the inactivation and recovery processes, an
observation that suggests that protein synthesis was not involved in
these processes. Our results suggest that osmotic stress decreased the amount of water in the cytoplasm via the efflux of water through water
channels (aquaporins), with resultant increases in intracellular concentrations of ions and a decrease in photosynthetic activity.
1
This work was financially supported in part by a
Grant-in-Aid for Specially Promoted Research (no. 08102011 to N.M.)
from the Ministry of Education, Science and Culture, Japan, and by the
National Institute for Basic Biology Cooperative Research Program on
the Stress Tolerance of Plants.
*
Corresponding author; e-mail murata{at}nibb.ac.jp; fax
81-564-54-4866.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
R. Vidal, L. Lopez-Maury, M. G. Guerrero, and F. J. Florencio
Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System
J. Bacteriol.,
July 1, 2009;
191(13):
4383 - 4391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sato, K. Nimura-Matsune, S. Watanabe, T. Chibazakura, and H. Yoshikawa
Expression Analysis of Multiple dnaK Genes in the Cyanobacterium Synechococcus elongatus PCC 7942
J. Bacteriol.,
May 15, 2007;
189(10):
3751 - 3758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Shapiguzov, A. A. Lyukevich, S. I. Allakhverdiev, T. V. Sergeyenko, I. Suzuki, N. Murata, and D. A. Los
Osmotic shrinkage of cells of Synechocystis sp. PCC 6803 by water efflux via aquaporins regulates osmostress-inducible gene expression
Microbiology,
February 1, 2005;
151(2):
447 - 455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Allakhverdiev, Y. Nishiyama, S. Takahashi, S. Miyairi, I. Suzuki, and N. Murata
Systematic Analysis of the Relation of Electron Transport and ATP Synthesis to the Photodamage and Repair of Photosystem II in Synechocystis
Plant Physiology,
January 1, 2005;
137(1):
263 - 273.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Kozono, X. Ding, I. Iwasaki, X. Meng, Y. Kamagata, P. Agre, and Y. Kitagawa
Functional Expression and Characterization of an Archaeal Aquaporin. AqpM FROM METHANOTHERMOBACTER MARBURGENSIS
J. Biol. Chem.,
March 14, 2003;
278(12):
10649 - 10656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Aharon, Y. Shahak, S. Wininger, R. Bendov, Y. Kapulnik, and G. Galili
Overexpression of a Plasma Membrane Aquaporin in Transgenic Tobacco Improves Plant Vigor under Favorable Growth Conditions but Not under Drought or Salt Stress
PLANT CELL,
February 1, 2003;
15(2):
439 - 447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Allakhverdiev, Y. Nishiyama, S. Miyairi, H. Yamamoto, N. Inagaki, Y. Kanesaki, and N. Murata
Salt Stress Inhibits the Repair of Photodamaged Photosystem II by Suppressing the Transcription and Translation of psbA Genes in Synechocystis
Plant Physiology,
November 1, 2002;
130(3):
1443 - 1453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Cruz, B. A. Salbilla, A. Kanazawa, and D. M. Kramer
Inhibition of Plastocyanin to P700+ Electron Transfer in Chlamydomonas reinhardtii by Hyperosmotic Stress
Plant Physiology,
November 1, 2001;
127(3):
1167 - 1179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Goh, R. Hedrich, and U. Schreiber
Osmotic Stress Induces Inactivation of Photosynthesis in Guard Cell Protoplasts of Vicia Leaves
Plant Cell Physiol.,
October 1, 2001;
42(10):
1186 - 1191.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Allakhverdiev, M. Kinoshita, M. Inaba, I. Suzuki, and N. Murata
Unsaturated Fatty Acids in Membrane Lipids Protect the Photosynthetic Machinery against Salt-Induced Damage in Synechococcus
Plant Physiology,
April 1, 2001;
125(4):
1842 - 1853.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. I. Allakhverdiev, A. Sakamoto, Y. Nishiyama, M. Inaba, and N. Murata
Ionic and Osmotic Effects of NaCl-Induced Inactivation of Photosystems I and II in Synechococcus sp.
Plant Physiology,
July 1, 2000;
123(3):
1047 - 1056.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Tchernov, Y. Helman, N. Keren, B. Luz, I. Ohad, L. Reinhold, T. Ogawa, and A. Kaplan
Passive Entry of CO2 and Its Energy-dependent Intracellular Conversion to HCO3- in Cyanobacteria Are Driven by a Photosystem I-generated Delta {micro}H+
J. Biol. Chem.,
June 22, 2001;
276(26):
23450 - 23455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
