|
Plant Physiol, April 2000, Vol. 122, pp. 1209-1216
Metabolic Relations of Inositol 3,4,5,6-Tetrakisphosphate
Revealed by Cell Permeabilization. Identification of Inositol
3,4,5,6-Tetrakisphosphate 1-Kinase and Inositol
3,4,5,6-Tetrakisphosphate Phosphatase Activities in Mesophyll
Cells1
Charles A.
Brearley* and
David E.
Hanke
Department of Plant Sciences, University of Cambridge, Downing
Street, Cambridge CB2 3EA, United Kingdom
Using a permeabilization strategy to
introduce Ins(3,4,5,6) P4 into mesophyll protoplasts of
Commelina communis, we have identified Ins(3,4,5,6)
P4 1-kinase activity in mesophyll cells. Multiple InsP3 isomers were identified in Spirodela
polyrhiza and Arabidopsis. Only two of these, Ins(1,2,3)
P3 and Ins(3,4,6) P3, have previously been
identified in plants and only in monocots. The isomers detected in
S. polyrhiza included D- and/or
L-Ins(3,4,5) P3, D- and/or L-Ins(3,5,6) P3, and D- and/or
L-Ins(2,4,5) P3. Ins(1,4,5) P3, if
present, was only a tiny fraction of total InsP3 species.
We have also identified inositol polyphosphate phosphatase
activities, Ins(3,4,5,6) P4 6-phosphatase and Ins(3,4,5, 6)
P4 4-phosphatase, whose action on endogenous inositol
polyphosphates explains the presence of D- and/or
L-Ins(3,4,5) P3 and D- and/or
L-Ins(3,5,6) P3 in mesophyll cells. Inositol
trisphosphates identified in Arabidopsis include Ins(1,2,3)
P3 and D- and/or L-Ins(3,4,6)
P3, suggesting that dicots may share pathways of
InsP6 biosynthesis and breakdown in common with monocots.
1
This work was supported by grants from the
Biotechnology and Biological Science Research Council (BBSRC) UK and
the Agricultural and Food Research Council UK to D.H. C.B. is a BBSRC
Advanced Research Fellow.
*
Corresponding author; e-mail cab1000{at}cus.cam.ac.uk; fax
44-1223-333953.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
S. Schneider, D. Beyhl, R. Hedrich, and N. Sauer
Functional and Physiological Characterization of Arabidopsis INOSITOL TRANSPORTER1, a Novel Tonoplast-Localized Transporter for myo-Inositol
PLANT CELL,
April 1, 2008;
20(4):
1073 - 1087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sun, M. Thompson, G. Lin, H. Butler, Z. Gao, S. Thornburgh, K. Yau, D. A. Smith, and V. K. Shukla
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase from Maize: Molecular and Biochemical Characterization
Plant Physiology,
July 1, 2007;
144(3):
1278 - 1291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mitsuhashi, M. Ohnishi, Y. Sekiguchi, Y.-U. Kwon, Y.-T. Chang, S.-K. Chung, Y. Inoue, R. J. Reid, H. Yagisawa, and T. Mimura
Phytic Acid Synthesis and Vacuolar Accumulation in Suspension-Cultured Cells of Catharanthus roseus Induced by High Concentration of Inorganic Phosphate and Cations
Plant Physiology,
July 1, 2005;
138(3):
1607 - 1614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-J. Xia, C. Brearley, S. Elge, B. Kaplan, H. Fromm, and B. Mueller-Roeber
Arabidopsis Inositol Polyphosphate 6-/3-Kinase Is a Nuclear Protein That Complements a Yeast Mutant Lacking a Functional ArgR-Mcm1 Transcription Complex
PLANT CELL,
February 1, 2003;
15(2):
449 - 463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zonia, S. Cordeiro, J. Tupy, and J. A. Feijo
Oscillatory Chloride Efflux at the Pollen Tube Apex Has a Role in Growth and Cell Volume Regulation and Is Targeted by Inositol 3,4,5,6-Tetrakisphosphate
PLANT CELL,
September 1, 2002;
14(9):
2233 - 2249.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Y. Perera, J. Love, I. Heilmann, W. F. Thompson, and W. F. Boss
Up-Regulation of Phosphoinositide Metabolism in Tobacco Cells Constitutively Expressing the Human Type I Inositol Polyphosphate 5-Phosphatase
Plant Physiology,
August 1, 2002;
129(4):
1795 - 1806.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Lemtiri-Chlieh, E. A. C. MacRobbie, and C. A. Brearley
Inositol hexakisphosphate is a physiological signal regulating the K+-inward rectifying conductance in guard cells
PNAS,
July 5, 2000;
(2000)
140217497.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Lemtiri-Chlieh, E. A. C. MacRobbie, and C. A. Brearley
From the Cover: Inositol hexakisphosphate is a physiological signal regulating the K+-inward rectifying conductance in guard cells
PNAS,
July 18, 2000;
97(15):
8687 - 8692.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
