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Plant Physiol, April 2000, Vol. 122, pp. 1217-1224
Is There a Role for Oligosaccharides in Seed Longevity? An
Assessment of Intracellular Glass Stability1
Julia
Buitink,*
Marcus A.
Hemminga, and
Folkert A.
Hoekstra
Laboratory of Molecular Physics, Dreijenlaan 3, 6703 HA Wageningen,
The Netherlands (J.B., M.A.H.); and Wageningen Agricultural University,
Laboratory of Plant Physiology, Arboretumlaan 4, 6703 BD
Wageningen, The Netherlands (J.B., F.A.H.)
We examined whether oligosaccharides
extend seed longevity by increasing the intracellular glass stability.
For that purpose, we used a spin probe technique to measure the
molecular mobility and glass transition temperature of the cytoplasm of
impatiens (Impatiens walleriana) and bell pepper
(Capsicum annuum) seeds that were osmo-primed to change
oligosaccharide content and longevity. Using saturation transfer
electron paramagnetic resonance spectroscopy, we found that the
rotational correlation time of the polar spin probe 3-carboxy-proxyl in
the cytoplasm decreased, together with longevity, as a function of
increasing seed water content, suggesting that longevity may indeed be
regulated by cytoplasmic mobility. Osmo-priming of the seeds resulted
in considerable decreases in longevity and oligosaccharide content,
while the sucrose content increased. No difference in the glass
transition temperature was found between control and primed impatiens
seeds at the same temperature and water content. Similarly, there was
no difference in the rotational motion of the spin probe in the
cytoplasm between control and primed impatiens and bell pepper seeds.
We therefore conclude that oligosaccharides in seeds do not affect the
stability of the intracellular glassy state, and that the reduced
longevity after priming is not the result of increased molecular
mobility in the cytoplasm.
1
This research was financially supported by the
Netherlands Technology Foundation (STW), and was coordinated by the
Life Sciences Foundation.
*
Corresponding author; e-mail julia.buitink{at}algem.pf.wau.nl; fax
31-317-484740.
© 2000 American Society of Plant Physiologists
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