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Plant Physiol, April 2000, Vol. 122, pp. 1249-1260
The Arabidopsis HKT1 Gene Homolog Mediates Inward
Na+ Currents in Xenopus laevis Oocytes and
Na+ Uptake in Saccharomyces
cerevisiae1
Nobuyuki
Uozumi,
Eugene J.
Kim,
Francisco
Rubio,2
Takao
Yamaguchi,
Shoshi
Muto,
Akio
Tsuboi,
Evert P.
Bakker,
Tatsunosuke
Nakamura, and
Julian I.
Schroeder
Bioscience Center, Nagoya University, Nagoya, 464-8601,
Japan (N.U., S.M.); Department of Biology and Center for Molecular
Genetics, University of California at San Diego, La Jolla, California
92093-0116 (N.U., E.J.K., F.R., J.I.S.); Graduate School of
Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan
(N.U., T.Y., S.M.); Department of Biophysics and Biochemistry, Graduate
School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo
113, Japan (A.T.); Abteilung Mikrobiologie, Universität
Osnabrück, Barbarastrasse 11, D-49076 Osnabrück, Germany
(E.P.B.); and Faculty of Pharmaceutical Sciences, Chiba University,
1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan (T.N.)
The
Na+-K+ co-transporter HKT1, first isolated from
wheat, mediates high-affinity K+ uptake. The function of
HKT1 in plants, however, remains to be elucidated, and the isolation of
HKT1 homologs from Arabidopsis would further studies of the roles of
HKT1 genes in plants. We report here the isolation of a cDNA homologous
to HKT1 from Arabidopsis (AtHKT1) and the
characterization of its mode of ion transport in heterologous systems.
The deduced amino acid sequence of AtHKT1 is 41% identical to that of
HKT1, and the hydropathy profiles are very similar.
AtHKT1 is expressed in roots and, to a lesser extent, in
other tissues. Interestingly, we found that the ion transport
properties of AtHKT1 are significantly different from the wheat
counterpart. As detected by electrophysiological measurements, AtHKT1
functioned as a selective Na+ uptake transporter in
Xenopus laevis oocytes, and the presence of external
K+ did not affect the AtHKT1-mediated ion conductance
(unlike that of HKT1). When expressed in Saccharomyces
cerevisiae, AtHKT1 inhibited growth of the yeast in a medium
containing high levels of Na+, which correlates to the
large inward Na+ currents found in the oocytes.
Furthermore, in contrast to HKT1, AtHKT1 did not complement the growth
of yeast cells deficient in K+ uptake when cultured in
K+-limiting medium. However, expression of
AtHKT1 did rescue Escherichia coli
mutants carrying deletions in K+ transporters. The rescue
was associated with a less than 2-fold stimulation of K+
uptake into K+-depleted cells. These data demonstrate that
AtHKT1 differs in its transport properties from the wheat HKT1,
and that AtHKT1 can mediate Na+ and, to a small degree,
K+ transport in heterologous expression systems.
1
This work was supported by the U.S. Department
of Agriculture (grant no. 98-3504-6684 to J.I.S.), by Grants-in-Aid
for Scientific Research from the Ministry of Education, Science, Sports
and Culture of Japan (nos. 11660082 and 11132227 to N.U.), and by the
Salt Science Research Foundation Grant. This work was also supported in
part by a U.S. Department of Agriculture Postdoctoral Fellowship (no.
9801009 to E.J.K.), by the Deutshe Forschungsgemeinschaft (no. SFB431
to E.P.B), and by the Fonds der Chemischen Industrie (to E.P.B.).
2
Present address: Departemento de Biotecnologia,
Escuela Tecnica Superior de Ingenierous Agronomos, 28040 Madrid, Spain.
© 2000 American Society of Plant Physiologists
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