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Plant Physiol, April 2000, Vol. 122, pp. 1335-1342
Expression of Allene Oxide Synthase Determines Defense Gene
Activation in Tomato1
Sobhana
Sivasankar,2*
Bay
Sheldrick, and
Steven
J.
Rothstein2
Department of Molecular Biology and Genetics, University of Guelph,
Guelph, Ontario, Canada N1G 2W1
Allene
oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes
the first step in the biosynthesis of jasmonic acid from
lipoxygenase-derived hydroperoxides of free fatty acids. Using the
AOS cDNA from tomato (Lycopersicon
esculentum), in which the role of jasmonic acid in
wound-induced defense gene activation has been best described, we
examined the kinetics of AOS induction in response to wounding and
elicitors, in parallel with that of the wound-inducible PIN
II (proteinase inhibitor II) gene. AOS was
induced in leaves by wounding, systemin, 12-oxophytodienoic acid, and
methyl jasmonate. The levels of AOS mRNA started
declining by 4 h after induction, whereas the levels of PIN
II mRNA continued to increase up to 20 h after induction.
Salicylic acid inhibited AOS and PIN II
expression, and the addition of 12-oxophytodienoic acid or methyl
jasmonate did not prevent the inhibition of PIN II
expression in the presence of salicylic acid. Ethylene induced the
expression of AOS, but the presence of ethylene alone
did not produce an optimal induction of PIN II. The
addition of silver thiosulfate, an ethylene action inhibitor, prevented
the wound-induced expression of both AOS and PIN
II. Products of hydroperoxide lyase affected neither
AOS nor PIN II, but induced expression of
prosystemin. Based on these results, we propose an updated model for
defense gene activation in tomato.
1
This work was supported by the Natural Sciences
and Engineering Research Council of Canada.
2
Present address: Pioneer Hi-Bred International,
Inc., Trait and Technology Development, 7250 NW 62nd Avenue, P.O. Box
552, Johnston, IA 50131-0552.
*
Corresponding author; e-mail sivasasobh{at}phibred.com; fax
515-334-4788.
© 2000 American Society of Plant Physiologists
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