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Plant Physiol, May 2000, Vol. 123, pp. 201-214

Internal Conductance to CO2 Diffusion and C18OO Discrimination in C3 Leaves1

Jim S. Gillon and Dan Yakir*

Department of Environmental Science and Energy Research, Weizmann Institute of Science, 76100 Rehovot, Israel

18O discrimination in CO2 stems from the oxygen exchange between 18O-enriched water and CO2 in the chloroplast, a process catalyzed by carbonic anhydrase (CA). A proportion of this 18O-labeled CO2 escapes back to the atmosphere, resulting in an effective discrimination against C18OO during photosynthesis (Delta 18O). By constraining the delta 18O of chloroplast water (delta e) by analysis of transpired water and the extent of CO2-H2O isotopic equilibrium (theta eq) by measurements of CA activity (theta eq = 0.75-1.0 for tobacco, soybean, and oak), we could apply measured Delta 18O in a leaf cuvette attached to a mass spectrometer to derive the CO2 concentration at the physical limit of CA activity, i.e. the chloroplast surface (ccs). From the CO2 drawdown sequence between stomatal cavities from gas exchange (ci), from Delta 18O (ccs), and at Rubisco sites from Delta 13C (cc), the internal CO2 conductance (gi) was partitioned into cell wall (gw) and chloroplast (gch) components. The results indicated that gch is variable (0.42-1.13 mol m-2 s-1) and proportional to CA activity. We suggest that the influence of CA activity on the CO2 assimilation rate should be important mainly in plants with low internal conductances.

1 This research was supported by the Israel Science Foundation (grant no. 308/96). J.S.G. was supported by a fellowship from the Leverhulme Trust, UK (no. SAS/30317) while at the Weizmann Institute of Science, and by the Natural Environment Research Council, UK (grant no. GT4/94/379) while at University of Newcastle upon Tyne, Department of Agriculture and Environmental Sciences, Ridley Building, Newcastle upon Tyne, UK.

* Corresponding author; e-mail ciyakir{at}wisemail.weizmann.ac.il; fax 972-8-934-4124.

© 2000 American Society of Plant Physiologists


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