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Plant Physiol, May 2000, Vol. 123, pp. 223-234
Expression of Spinach Ascorbate Peroxidase Isoenzymes in
Response to Oxidative Stresses1
Kazuya
Yoshimura,
Yukinori
Yabuta,
Takahiro
Ishikawa,2 and
Shigeru
Shigeoka*
Department of Food and Nutrition, Faculty of Agriculture, Kinki
University, 3327-204 Nakamachi, Nara 631-8505, Japan
We studied the response of each ascorbate peroxidase (APX)
isoenzyme in spinach leaves under stress conditions imposed by high
light intensity, drought, salinity, and applications of methyl viologen and abscisic acid. The steady-state transcript level of
cytosolic APX remarkably increased in response to high-light stress and
methyl viologen treatment, but not in response to the other stress
treatments. The transcript levels of the chloroplastic (stromal and
thylakoid-bound) and microbody-bound APX isoenzymes were not changed in
response to any of the stress treatments. To explore the responses of
the APX isoenzymes to photooxidative stress, the levels of transcript
and protein and activities of each isoenzyme were studied during
high-light stress and following its recovery. The cytosolic APX
activity increased in parallel with transcript abundance during
high-light stress, while the protein level was not altered. The other
isoenzymes showed no significant changes in transcript and protein
levels and activities, except for the gradual decrease in chloroplastic
isoenzyme activities.
1
This work was supported by a Grant-in-Aid for
Scientific Research (no. 10660102 to S.S.) from the Ministry of
Education, Science, Sports and Culture, Japan.
2
Present address: Faculty of Life and
Environmental Science, Simane University, 1060 Nishikawatsu, Matsue,
Shimane 690-8504, Japan.
*
Corresponding author; e-mail shigeoka{at}nara.kindai.ac.jp; fax
81-742-43-2252.
© 2000 American Society of Plant Physiologists
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