|
Plant Physiol, May 2000, Vol. 123, pp. 243-254
Comparative Analysis of Expressed Sequences in Phytophthora
sojae1
Dinah
Qutob,
Peter T.
Hraber,
Bruno W.S.
Sobral, and
Mark
Gijzen*
Agriculture and Agri-Food Canada, 1391 Sandford Street, London,
Ontario, Canada N5V 4T3 (D.Q., M.G.); Department of Microbiology and
Immunology, University of Western Ontario, London, Ontario, Canada N6A
5C1 (D.Q.); and National Center for Genome Resources, 1800 Old Pecos
Trail, Santa Fe, New Mexico 87505 (P.T.H., B.W.S.S.)
Phytophthora sojae (Kaufmann and Gerdemann) is an
oomycete that causes stem and root rot on soybean (Glycine
max L. Merr) plants. We have constructed three cDNA libraries
using mRNA isolated from axenically grown mycelium and zoospores and
from tissue isolated from plant hypocotyls 48 h after inoculation
with zoospores. A total of 3,035 expressed sequence tags (ESTs) were
generated from the three cDNA libraries, representing an estimated
2,189 cDNA transcripts. The ESTs were classified according to putative
function based on similarity to known proteins, and were analyzed for
redundancy within and among the three source libraries. Distinct
expression patterns were observed for each library. By analysis of the
percentage G+C content of the ESTs, we estimate that two-thirds of the
ESTs from the infected plant library are derived from P.
sojae cDNA transcripts. The ESTs originating from this study
were also compared with a collection of Phytophthora
infestans ESTs and with all other non-human ESTs to assess the
similarity of the P. sojae sequences to existing EST
data. This collection of cDNA libraries, ESTs, and accompanying
annotation will provide a new resource for studies on oomycetes and on
soybean responses to pathogen challenge.
1
This work was supported in part by a grant from
the Ontario Soybean Growers.
*
Corresponding author; e-mail gijzenm{at}em.agr.ca; fax
519-457-3997.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
H. Chen, P. Seguin, A. Archambault, L. Constan, and S. Jabaji
Gene Expression and Isoflavone Concentrations in Soybean Sprouts Treated with Chitosan
Crop Sci.,
January 28, 2009;
49(1):
224 - 236.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Savidor, R. S. Donahoo, O. Hurtado-Gonzales, M. L. Land, M. B. Shah, K. H. Lamour, and W. H. McDonald
Cross-species Global Proteomics Reveals Conserved and Unique Processes in Phytophthora sojae and Phytophthora ramorum
Mol. Cell. Proteomics,
August 1, 2008;
7(8):
1501 - 1516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Y. Jiang, B. M. Tyler, S. C. Whisson, A. R. Hardham, and F. Govers
Ancient Origin of Elicitin Gene Clusters in Phytophthora Genomes
Mol. Biol. Evol.,
February 1, 2006;
23(2):
338 - 351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dhaubhadel, K. Kuflu, M. C. Romero, and M. Gijzen
A soybean seed protein with carboxylate-binding activity
J. Exp. Bot.,
September 1, 2005;
56(419):
2335 - 2344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rudd and I. V. Tetko
Eclair--a web service for unravelling species origin of sequences sampled from mixed host interfaces
Nucleic Acids Res.,
July 1, 2005;
33(suppl_2):
W724 - W727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Jantasuriyarat, M. Gowda, K. Haller, J. Hatfield, G. Lu, E. Stahlberg, B. Zhou, H. Li, H. Kim, Y. Yu, et al.
Large-Scale Identification of Expressed Sequence Tags Involved in Rice and Rice Blast Fungus Interaction
Plant Physiology,
May 1, 2005;
138(1):
105 - 115.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Torto, S. Li, A. Styer, E. Huitema, A. Testa, N. A.R. Gow, P. van West, and S. Kamoun
EST Mining and Functional Expression Assays Identify Extracellular Effector Proteins From the Plant Pathogen Phytophthora
Genome Res.,
July 1, 2003;
13(7):
1675 - 1685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kamoun
Molecular Genetics of Pathogenic Oomycetes
Eukaryot. Cell,
April 1, 2003;
2(2):
191 - 199.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. C. Rose, K.-S. Ham, A. G. Darvill, and P. Albersheim
Molecular Cloning and Characterization of Glucanase Inhibitor Proteins: Coevolution of a Counterdefense Mechanism by Plant Pathogens
PLANT CELL,
June 1, 2002;
14(6):
1329 - 1345.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Dacks and W. F. Doolittle
Novel syntaxin gene sequences from Giardia, Trypanosoma and algae: implications for the ancient evolution of the eukaryotic endomembrane system
J. Cell Sci.,
April 15, 2002;
115(8):
1635 - 1642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. MacGregor, M. Bhattacharyya, B. Tyler, R. Bhat, A. F. Schmitthenner, and M. Gijzen
Genetic and Physical Mapping of Avr1a in Phytophthora sojae
Genetics,
March 1, 2002;
160(3):
949 - 959.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. G. Andersson and L. Cerenius
Pumilio Homologue from Saprolegnia parasitica Specifically Expressed in Undifferentiated Spore Cysts
Eukaryot. Cell,
February 1, 2002;
1(1):
105 - 111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gijzen, K. Kuflu, D. Qutob, and J. T. Chernys
A class I chitinase from soybean seed coat
J. Exp. Bot.,
December 1, 2001;
52(365):
2283 - 2289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kamoun and S. A. Hogenhout
Agricultural Microbes Genome 2: First Glimpses into the Genomes of Plant-Associated Microbes
PLANT CELL,
March 1, 2001;
13(3):
451 - 458.
[Full Text]
|
|
|
|
|
