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Plant Physiol, May 2000, Vol. 123, pp. 307-318
Regulation of High-Affinity Nitrate Transporter Genes and
High-Affinity Nitrate Influx by Nitrogen Pools in Roots of
Barley1
Joseph John
Vidmar,
Degen
Zhuo,
M. Yaeesh
Siddiqi,
Jan K.
Schjoerring,
Bruno
Touraine, and
Anthony D.M.
Glass*
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, British Columbia, Canada V6T 1Z4
(J.J.V., D.Z., M.Y.S., A.D.M.G.); Plant Nutrition Laboratory,
Department of Agricultural Sciences, Royal Veterinary and Agricultural
University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen,
Denmark (J.K.S.); and Biochimie et Physiologie Moléculaire des
Plantes, Ecole Nationale Supérieure Agronomique de
Montpellier/Institut National de la Recherche Agronomique/Centre
National de la Recherche Scientifique Unité de Recherche
Associée 2133, 34060 Montpellier cedex 1, France (B.T.)
To investigate the regulation of HvNRT2, genes that
encode high-affinity NO3 transporters in
barley (Hordeum vulgare) roots, seedlings were treated
with 10 mM NO3 in the presence or
absence of amino acids (aspartate, asparagine, glutamate [Glu], and
glutamine [Gln]), NH4+, and/or inhibitors of
N assimilation. Although all amino acids decreased high-affinity
13NO3 influx and
HvNRT2 transcript abundance, there was substantial interconversion of administered amino acids, making it impossible to
determine which amino acid(s) were responsible for the observed effects. To clarify the role of individual amino acids, plants were
separately treated with tungstate, methionine sulfoximine, or azaserine
(inhibitors of nitrate reductase, Gln synthetase, and Glu synthase,
respectively). Tungstate increased the HvNRT2 transcript
by 20% to 30% and decreased NO3 influx by
50%, indicating that NO3 itself does not
regulate transcript abundance, but may exert post-transcriptional
effects. Experiments with methionine sulfoximine suggested that
NH4+ may down-regulate HvNRT2
gene expression and high-affinity NO3 influx
by effects operating at the transcriptional and post-transcriptional levels. Azaserine decreased HvNRT2 transcript levels and
NO3 influx by 97% and 95%, respectively,
while decreasing Glu and increasing Gln levels. This suggests that Gln
(and not Glu) is responsible for down-regulating HvNRT2
expression, although it does not preclude a contributory effect of
other amino acids.
1
This work was supported by Natural Sciences and
Engineering Research Council of Canada Strategic and Research grants
(to A.D.M.G.).
*
Corresponding author; e-mail aglass{at}unixg.ubc.ca; fax
604-822-6089.
© 2000 American Society of Plant Physiologists
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