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Plant Physiol, May 2000, Vol. 123, pp. 319-326
Mutation of Arabidopsis Plastid Phosphoglucose Isomerase Affects
Leaf Starch Synthesis and Floral Initiation1
Tien-Shin
Yu,
Wei-Ling
Lue,
Shue-Mei
Wang,2 and
Jychian
Chen2*
Graduate Institute of Life Science, National Defense
Medical Center, Taipei 114, Taiwan, Republic of China (T.-S.Y.);
Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan,
Republic of China (T.-S.Y., W.-L.L., J.C.); and Department of Botany,
National Taiwan University, Taipei 106, Taiwan, Republic of China
(S.-M.W.)
We isolated pgi1-1, an Arabidopsis mutant with a
decreased plastid phospho-glucose (Glc) isomerase activity. While
pgi1-1 mutant has a deficiency in leaf starch synthesis,
it accumulates starch in root cap cells. It has been shown that a
plastid transporter for hexose phosphate transports cytosolic Glc-6-P
into plastids and expresses restricted mainly to the heterotrophic
tissues. The decreased starch content in leaves of the
pgi1-1 mutant indicates that cytosolic Glc-6-P cannot be
efficiently transported into chloroplasts to complement the mutant's
deficiency in chloroplastic phospho-Glc isomerase activity for starch
synthesis. We cloned the Arabidopsis PGI1 gene and showed that it
encodes the plastid phospho-Glc isomerase. The pgi1-1
allele was found to have a single nucleotide substitution, causing a
Ser to Phe transition. While the flowering times of the Arabidopsis
starch-deficient mutants pgi1, pgm1, and
adg1 were similar to that of the wild type under long-day conditions, it was significantly delayed under short-day conditions. The pleiotropic phenotype of late flowering conferred by
these starch metabolic mutations suggests that carbohydrate metabolism
plays an important role in floral initiation.
1
This work was supported by the National Science
Council (Taiwan, Republic of China; grant nos. NSC
88-2311-B-002-030 to S.-M.W. and NSC 87-2311-B-001-074 to J.C.)
and by Academia Sinica (to J.C.).
2
These authors contributed equally to the paper.
*
Corresponding author; e-mail mbjchen{at}ccvax.sinica.edu.tw;
fax 8862-27899208.
© 2000 American Society of Plant Physiologists
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