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Plant Physiol, May 2000, Vol. 123, pp. 319-326

Mutation of Arabidopsis Plastid Phosphoglucose Isomerase Affects Leaf Starch Synthesis and Floral Initiation1

Tien-Shin Yu, Wei-Ling Lue, Shue-Mei Wang,2 and Jychian Chen2*

Graduate Institute of Life Science, National Defense Medical Center, Taipei 114, Taiwan, Republic of China (T.-S.Y.); Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China (T.-S.Y., W.-L.L., J.C.); and Department of Botany, National Taiwan University, Taipei 106, Taiwan, Republic of China (S.-M.W.)

We isolated pgi1-1, an Arabidopsis mutant with a decreased plastid phospho-glucose (Glc) isomerase activity. While pgi1-1 mutant has a deficiency in leaf starch synthesis, it accumulates starch in root cap cells. It has been shown that a plastid transporter for hexose phosphate transports cytosolic Glc-6-P into plastids and expresses restricted mainly to the heterotrophic tissues. The decreased starch content in leaves of the pgi1-1 mutant indicates that cytosolic Glc-6-P cannot be efficiently transported into chloroplasts to complement the mutant's deficiency in chloroplastic phospho-Glc isomerase activity for starch synthesis. We cloned the Arabidopsis PGI1 gene and showed that it encodes the plastid phospho-Glc isomerase. The pgi1-1 allele was found to have a single nucleotide substitution, causing a Ser to Phe transition. While the flowering times of the Arabidopsis starch-deficient mutants pgi1, pgm1, and adg1 were similar to that of the wild type under long-day conditions, it was significantly delayed under short-day conditions. The pleiotropic phenotype of late flowering conferred by these starch metabolic mutations suggests that carbohydrate metabolism plays an important role in floral initiation.

1 This work was supported by the National Science Council (Taiwan, Republic of China; grant nos. NSC 88-2311-B-002-030 to S.-M.W. and NSC 87-2311-B-001-074 to J.C.) and by Academia Sinica (to J.C.).

2 These authors contributed equally to the paper.

* Corresponding author; e-mail mbjchen{at}ccvax.sinica.edu.tw; fax 8862-27899208.

© 2000 American Society of Plant Physiologists


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