Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (38)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gisbert, C.
Right arrow Articles by Moreno, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gisbert, C.
Right arrow Articles by Moreno, V.
Agricola
Right arrow Articles by Gisbert, C.
Right arrow Articles by Moreno, V.

Plant Physiol, May 2000, Vol. 123, pp. 393-402

The Yeast HAL1 Gene Improves Salt Tolerance of Transgenic Tomato1

Carmina Gisbert,* Ana M. Rus, M. Carmen Bolarín, J. Miguel López-Coronado, Isabel Arrillaga,2 Consuelo Montesinos, Manuel Caro, Ramon Serrano, and Vicente Moreno

Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Laboratorio Nine (C.G., I.A., V.M.), and Laboratorio Three (J.M.L.-C., C.M., R.S.) Camino de Vera, 14, Universidad Politécnica de Valencia, 46022-Valencia, Spain; and Centro de Edafología y Biología Aplicada del Segura, Consejo Superior de Investigaciones Científicas, Apartado 4195, Murcia E-30080, Spain (A.M.R., M.C.B., M.C.)

Overexpression of the HAL1 gene in yeast has a positive effect on salt tolerance by maintaining a high internal K+ concentration and decreasing intracellular Na+ during salt stress. In the present work, the yeast gene HAL1 was introduced into tomato (Lycopersicon esculentum Mill.) by Agrobacterium tumefaciens-mediated transformation. A sample of primary transformants was self-pollinated, and progeny from both transformed and non-transformed plants (controls) were evaluated for salt tolerance in vitro and in vivo. Results from different tests indicated a higher level of salt tolerance in the progeny of two different transgenic plants bearing four copies or one copy of the HAL1 gene. In addition, measurement of the intracellular K+ to Na+ ratios showed that transgenic lines were able to retain more K+ than the control under salt stress. Although plants and yeast cannot be compared in an absolute sense, these results indicate that the mechanism controlling the positive effect of the HAL1 gene on salt tolerance may be similar in transgenic plants and yeast.

1 This work was supported by the European Union (project no. AIR-3 CT94-1508).

2 Present address: Departamento de Fisiología Vegetal, Facultad de Farmacia, Campus de Burjassot, Universidad de Valencia, 46100 Burjassot, Spain.

* Corresponding author; e-mail cgisbert{at}ibmcp.upv.es; fax 34-96-3877859.

© 2000 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 jashsHome page
S. Zhou, R. Sauve, T. Fish, and T. W. Thannhauser
Salt-induced and Salt-suppressed Proteins in Tomato Leaves
J. Amer. Soc. Hort. Sci., March 1, 2009; 134(2): 289 - 294.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. R. Fulgenzi, M. L. Peralta, S. Mangano, C. H. Danna, A. J. Vallejo, P. Puigdomenech, and G. E. Santa-Maria
The Ionic Environment Controls the Contribution of the Barley HvHAK1 Transporter to Potassium Acquisition
Plant Physiology, May 1, 2008; 147(1): 252 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Chen, I. I. Pottosin, T. A. Cuin, A. T. Fuglsang, M. Tester, D. Jha, I. Zepeda-Jazo, M. Zhou, M. G. Palmgren, I. A. Newman, et al.
Root Plasma Membrane Transporters Controlling K+/Na+ Homeostasis in Salt-Stressed Barley
Plant Physiology, December 1, 2007; 145(4): 1714 - 1725.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Cuartero, M. C. Bolarin, M. J. Asins, and V. Moreno
Increasing salt tolerance in the tomato
J. Exp. Bot., March 1, 2006; 57(5): 1045 - 1058.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. J. Flowers
Improving crop salt tolerance
J. Exp. Bot., February 1, 2004; 55(396): 307 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. Gao, Z. Ren, Y. Zhao, and H. Zhang
Overexpression of SOD2 Increases Salt Tolerance of Arabidopsis
Plant Physiology, December 1, 2003; 133(4): 1873 - 1881.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Waditee, T. Hibino, T. Nakamura, A. Incharoensakdi, and T. Takabe
Overexpression of a Na+/H+ antiporter confers salt tolerance on a freshwater cyanobacterium, making it capable of growth in sea water
PNAS, March 19, 2002; 99(6): 4109 - 4114.
[Abstract] [Full Text] [PDF]

Home page
 Integr. Comp. Biol.Home page
J. C. Cushman
Osmoregulation in Plants: Implications for Agriculture
Integr. Comp. Biol., August 1, 2001; 41(4): 758 - 769.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. L. Koyama, A. Levesley, R. M.D. Koebner, T. J. Flowers, and A. R. Yeo
Quantitative Trait Loci for Component Physiological Traits Determining Salt Tolerance in Rice
Plant Physiology, January 1, 2001; 125(1): 406 - 422.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2000 by the American Society of Plant Biologists