|
Plant Physiol, May 2000, Vol. 123, pp. 51-58
Molecular Cloning and Targeting of a Fibrillarin Homolog
from Arabidopsis1
Kyoung Tae
Pih,2
Min Joo
Yi,
Ying Shi
Liang,
Bong Jeong
Shin,
Moo Je
Cho,
Inhwan
Hwang,2 and
Daeyoung
Son*
Department of Molecular Biology (M.J.Y., Y.S.L., B.J.S., I.H.,
D.S.), Plant Molecular Biology and Biotechnology Research Center
(K.T.P., M.J.C., I.H., D.S.), and Center for Plant Intracellular
Trafficking (K.T.P., I.H.), Gyeongsang National University, Chinju
660-701, Korea
Fibrillarin is a nucleolar protein known to be involved in the
processing of ribosomal RNA precursors. We isolated
AtFbr1, a cDNA encoding a homolog of fibrillarin in
Arabidopsis. The cDNA is 1.2 kb in size and encodes a polypeptide of
310 amino acid residues with a molecular mass of 33 kD.
AtFbr1 is expressed at high levels in the flower and
root tissue and at a slightly lower level in leaf tissue, whereas it
was nearly undetectable in siliques. Expression of
AtFbr1 was compared with that of the FLP
(fibrillarin-like protein) gene identified by the Arabidopsis genome
project. Abscisic acid treatment resulted in the down-regulation of the
expression of both AtFbr1 and FLP genes
in seedlings, although the degree of suppression was higher for
FLP than for AtFbr1. In addition, the
expression level of FLP decreased with the age of the
seedlings, whereas AtFbr1 did not exhibit any detectable
change. The subcellular localization of AtFbrl was studied with an in
vivo targeting approach using a fusion protein, and was found to be
correctly targeted to the nucleolus in protoplasts when expressed as a
green fluorescent fusion protein (GFP). Deletion experiments showed
that the N-terminal glycine- and arginine-rich region is necessary and
sufficient to target AtFbr1 to the nucleolus.
1
This work was supported by the Korea Research
Foundation made in the program year of 1998 (grant no.
1998-019-D00131).
2
Present address: Department of Life Science and
Center for Plant Intracellular Trafficking, Pohang University of
Science and Technology, Pohang 790-784, Korea.
*
Corresponding author; e-mail dyson{at}nongae.gsnu.ac.kr; fax
82-591-759-9363.
© 2000 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
W. Chi, J. Ma, D. Zhang, J. Guo, F. Chen, C. Lu, and L. Zhang
The Pentratricopeptide Repeat Protein DELAYED GREENING1 Is Involved in the Regulation of Early Chloroplast Development and Chloroplast Gene Expression in Arabidopsis
Plant Physiology,
June 1, 2008;
147(2):
573 - 584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Tillemans, I. Leponce, G. Rausin, L. Dispa, and P. Motte
Insights into Nuclear Organization in Plants as Revealed by the Dynamic Distribution of Arabidopsis SR Splicing Factors
PLANT CELL,
November 1, 2006;
18(11):
3218 - 3234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Leslie, W. Zhang, B. L. Timney, B. T. Chait, M. P. Rout, R. W. Wozniak, and J. D. Aitchison
Characterization of Karyopherin Cargoes Reveals Unique Mechanisms of Kap121p-Mediated Nuclear Import
Mol. Cell. Biol.,
October 1, 2004;
24(19):
8487 - 8503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. H. Lee, M. K. Min, Y. J. Lee, J. B. Jin, D. H. Shin, D. H. Kim, K.-H. Lee, and I. Hwang
ADP-Ribosylation Factor 1 of Arabidopsis Plays a Critical Role in Intracellular Trafficking and Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis
Plant Physiology,
August 1, 2002;
129(4):
1507 - 1520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. J. Lee, D. H. Kim, Y.-W. Kim, and I. Hwang
Identification of a Signal That Distinguishes between the Chloroplast Outer Envelope Membrane and the Endomembrane System in Vivo
PLANT CELL,
October 1, 2001;
13(10):
2175 - 2190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Snaar, Wiesmeijer, A. Jochemsen, H. Tanke, and R. Dirks
Mutational Analysis of Fibrillarin and Its Mobility in Living Human Cells
J. Cell Biol.,
October 30, 2000;
151(3):
653 - 662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
