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Plant Physiol, June 2000, Vol. 123, pp. 553-562
A Stress-Inducible Gene for 9-cis-Epoxycarotenoid Dioxygenase
Involved in Abscisic Acid Biosynthesis under Water Stress in
Drought-Tolerant Cowpea1
Satoshi
Iuchi,
Masatomo
Kobayashi,
Kazuko
Yamaguchi-Shinozaki, and
Kazuo
Shinozaki*
Laboratory of Plant Molecular Biology, RIKEN Isukuba Institute,
3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074 Japan (S.I., M.K., K.Y.-S.,
K.S.); and Biological Resources Division, Japan International Research
Center for Agricultural Sciences, Ministry of Agriculture, Forestry,
and Fisheries, 2-1 Ohwashi, Tsukuba, Ibaraki 305-0851 Japan
(K.Y.-S.)
Four cDNA clones named CPRD (cowpea responsive to dehydration)
corresponding to genes that are responsive to dehydration were isolated
using differential screening of a cDNA library prepared from 10-h
dehydrated drought-tolerant cowpea (Vigna unguiculata) plants. One of the cDNA clones has a homology to 9-cis-epoxycarotenoid dioxygenase (named VuNCED1), which is supposed to be
involved in abscisic acid (ABA) biosynthesis. The GST (glutathione
S-transferase)-fused protein indicates a
9-cis-epoxycarotenoid dioxygenase activity, which catalyzes the
cleavage of 9-cis-epoxycarotenoid. The N-terminal region of the VuNCED1
protein directed the fused sGFP (synthetic green-fluorescent protein)
into the plastids of the protoplasts, indicating that the N-terminal
sequence acts as a transit peptide. Both the accumulation of ABA and
expression of VuNCED1 were strongly induced by drought
stress in the 8-d-old cowpea plant, whereas drought stress did not
trigger the expression of VuABA1 (accession no.
AB030295) gene that encodes zeaxanthin epoxidase. These results
indicate that the VuNCED1 cDNA encodes a
9-cis-epoxycarotenoid dioxygenase and that its product has a key role
in the synthesis of ABA under drought stress.
1
This work was supported in part by the Program
for Promotion of Basic Research Activities for Innovative Biosciences,
the Special Coordination Fund of the Science and Technology Agency of
the Japanese Government, by a Grant-in-Aid from the Ministry of
Education, Science and Culture of Japan, and by the Special Postdoctoral Researchers Program from the Science and Technology Agency
of the Japanese Government (to S.I.).
*
Corresponding author; e-mail sinozaki{at}rtc.riken.go.jp; fax
81-298-36-9060.
© 2000 American Society of Plant Physiologists
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